Detailed information of TA system TA144168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3073948..3074653
• Replicon: chromosome
• Accession: NZ_CP046396
• Organism: Escherichia coli strain ampC_0069

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: GNX12_RS14575
• Protein ID: WP_000539521.1
• Coordinates: 3074267..3074653 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GNX12_RS14570
• Protein ID: WP_001280945.1
• Coordinates: 3073948..3074277 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNX12_RS14555	3069106..3070017	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
GNX12_RS14560	3070194..3072542	+	2349	WP_022645413.1	EAL domain-containing protein	-
GNX12_RS14565	3072550..3073878	+	1329	WP_022645414.1	GGDEF domain-containing protein	-
GNX12_RS14570	3073948..3074277	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
GNX12_RS14575	3074267..3074653	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GNX12_RS14580	3074879..3076204	-	1326	WP_129666835.1	30S ribosomal protein S12 methylthiotransferase RimO	-
GNX12_RS14585	3076417..3076800	+	384	WP_000497137.1	biofilm formation regulator BssR	-
GNX12_RS14590	3076911..3078026	+	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
GNX12_RS14595	3078023..3078649	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T144168 WP_000539521.1 NZ_CP046396:c3074653-3074267 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T144168 NZ_CP061758:c1179512-1179135 [Escherichia coli O68:H12]
ATGCAAACCCAACCACTATCCTCAACTCAGGAGGCTACACCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGGGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT144168 WP_001280945.1 NZ_CP046396:c3074277-3073948 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT144168 NZ_CP061758:c1179922-1179563 [Escherichia coli O68:H12]
TTGTCGAATGAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTACAGGCGGGCAACCGTCTGCATTATCTCGCTGACCGGGCCAACATCACCGGACAGTTCAGCGACG
CTGATTTACGGTATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAAT
CCCTGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6