Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1000217..1000871 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GNX12_RS04765 Protein ID WP_000244781.1
Coordinates 1000464..1000871 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag GNX12_RS04760 Protein ID WP_001564007.1
Coordinates 1000217..1000483 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS04740 996305..997738 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
GNX12_RS04745 997783..998094 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
GNX12_RS04750 998258..998917 + 660 WP_000250274.1 hemolysin III family protein -
GNX12_RS04755 998994..999974 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
GNX12_RS04760 1000217..1000483 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
GNX12_RS04765 1000464..1000871 + 408 WP_000244781.1 protein YgfX Toxin
GNX12_RS04770 1000911..1001432 - 522 WP_001055874.1 flavodoxin FldB -
GNX12_RS04775 1001544..1002440 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GNX12_RS04780 1002465..1003175 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GNX12_RS04785 1003181..1004914 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T144155 WP_000244781.1 NZ_CP046396:1000464-1000871 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T144155 NZ_CP061754:2813364-2813471 [Escherichia coli O18ac:H14]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCCGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT144155 WP_001564007.1 NZ_CP046396:1000217-1000483 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT144155 NZ_CP061754:c2813307-2813251 [Escherichia coli O18ac:H14]
TCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGGTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References