Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 852183..853015 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name yeeV Uniprot ID A0A6G4K9Y9
Locus tag GNX12_RS03990 Protein ID WP_032333762.1
Coordinates 852183..852557 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NQ68
Locus tag GNX12_RS03995 Protein ID WP_001540478.1
Coordinates 852647..853015 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS03960 847296..848444 - 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -
GNX12_RS03965 848516..849499 - 984 WP_024946586.1 KpsF/GutQ family sugar-phosphate isomerase -
GNX12_RS03970 850310..850465 - 156 WP_000729638.1 hypothetical protein -
GNX12_RS03975 850823..851392 - 570 WP_001560692.1 DUF4942 domain-containing protein -
GNX12_RS03980 851489..851686 - 198 WP_000839281.1 DUF957 domain-containing protein -
GNX12_RS03985 851698..852186 - 489 WP_000777545.1 hypothetical protein -
GNX12_RS03990 852183..852557 - 375 WP_032333762.1 TA system toxin CbtA family protein Toxin
GNX12_RS03995 852647..853015 - 369 WP_001540478.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GNX12_RS04000 853178..853399 - 222 WP_000692311.1 DUF987 domain-containing protein -
GNX12_RS04005 853462..853938 - 477 WP_001186786.1 RadC family protein -
GNX12_RS04010 853954..854439 - 486 WP_000214398.1 antirestriction protein -
GNX12_RS04015 854530..855348 - 819 WP_001773857.1 DUF945 domain-containing protein -
GNX12_RS04020 855438..855671 - 234 WP_001278283.1 DUF905 family protein -
GNX12_RS04025 855677..856354 - 678 WP_001097301.1 hypothetical protein -
GNX12_RS04030 856502..857182 - 681 WP_032333197.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papX 850696..924149 73453


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14017.06 Da        Isoelectric Point: 8.2905

>T144154 WP_032333762.1 NZ_CP046396:c852557-852183 [Escherichia coli]
MKTLPDIHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T144154 NZ_CP061754:2812828-2812935 [Escherichia coli O18ac:H14]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13652.53 Da        Isoelectric Point: 7.8398

>AT144154 WP_001540478.1 NZ_CP046396:c853015-852647 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT144154 NZ_CP061754:c2812772-2812716 [Escherichia coli O18ac:H14]
TCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4K9Y9

Antitoxin

Source ID Structure
AlphaFold DB S1NQ68

References