Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 166874..167486 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag GNX12_RS00705 Protein ID WP_000833473.1
Coordinates 166874..167059 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag GNX12_RS00710 Protein ID WP_022646255.1
Coordinates 167076..167486 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS00690 162340..163635 + 1296 WP_000985736.1 Fic family protein -
GNX12_RS00695 163743..165281 + 1539 WP_000183976.1 aldehyde dehydrogenase AldB -
GNX12_RS00700 165322..166401 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
GNX12_RS00705 166874..167059 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
GNX12_RS00710 167076..167486 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GNX12_RS00715 167598..169577 - 1980 WP_022646254.1 glycoside hydrolase family 127 protein -
GNX12_RS00720 169588..170988 - 1401 WP_001600688.1 MFS transporter -
GNX12_RS00725 171214..172029 + 816 WP_022646253.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T144152 WP_000833473.1 NZ_CP046396:166874-167059 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T144152 NZ_CP061754:2523817-2525139 [Escherichia coli O18ac:H14]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGATTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT144152 WP_022646255.1 NZ_CP046396:167076-167486 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT144152 NZ_CP061754:2523551-2523817 [Escherichia coli O18ac:H14]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References