Detailed information of TA system TA144151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2599015..2599666
• Replicon: chromosome
• Accession: NZ_CP046395
• Organism: Lactobacillus rhamnosus GG strain JL-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: GM657_RS12135
• Protein ID: WP_005686631.1
• Coordinates: 2599015..2599398 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: GM657_RS12140
• Protein ID: WP_005686632.1
• Coordinates: 2599418..2599666 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM657_RS12125	2598051..2598578	-	528	WP_005686630.1	QueT transporter family protein	-
GM657_RS12130	2598616..2598813	-	198	WP_064536013.1	hypothetical protein	-
GM657_RS12135	2599015..2599398	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GM657_RS12140	2599418..2599666	-	249	WP_005686632.1	antitoxin	Antitoxin
GM657_RS12145	2599778..2600917	-	1140	WP_005686633.1	alanine racemase	-
GM657_RS12150	2600904..2601278	-	375	WP_005686634.1	holo-ACP synthase	-
GM657_RS12155	2601447..2602955	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
GM657_RS12160	2603229..2604617	-	1389	WP_015765066.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T144151 WP_005686631.1 NZ_CP046395:c2599398-2599015 [Lactobacillus rhamnosus GG]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T144151 NZ_CP061754:2135968-2136071 [Escherichia coli O18ac:H14]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT144151 WP_005686632.1 NZ_CP046395:c2599666-2599418 [Lactobacillus rhamnosus GG]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT144151 NZ_CP061754:c2136109-2135838 [Escherichia coli O18ac:H14]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19