Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4066008..4066627 Replicon chromosome
Accession NZ_CP046381
Organism Klebsiella pneumoniae strain BD_DM_782

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GM011_RS19720 Protein ID WP_002892050.1
Coordinates 4066409..4066627 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GM011_RS19715 Protein ID WP_002892066.1
Coordinates 4066008..4066382 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GM011_RS19705 4061160..4062353 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GM011_RS19710 4062376..4065522 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GM011_RS19715 4066008..4066382 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GM011_RS19720 4066409..4066627 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GM011_RS19725 4066786..4067352 + 567 WP_002892030.1 maltose O-acetyltransferase -
GM011_RS28725 4067324..4067464 - 141 WP_004147370.1 hypothetical protein -
GM011_RS19730 4067485..4067955 + 471 WP_002892026.1 YlaC family protein -
GM011_RS19735 4067930..4069381 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
GM011_RS19740 4069482..4070180 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
GM011_RS19745 4070177..4070317 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GM011_RS19750 4070317..4070580 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T144142 WP_002892050.1 NZ_CP046381:4066409-4066627 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T144142 NZ_CP061751:26834-27214 [Escherichia coli O15:H12]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT144142 WP_002892066.1 NZ_CP046381:4066008-4066382 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT144142 NZ_CP061751:26613-26834 [Escherichia coli O15:H12]
ATGCAATCCATTAACTTCCGTACCGCGCGGGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References