Detailed information of TA system TA144140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2663091..2664679
• Replicon: chromosome
• Accession: NZ_CP046381
• Organism: Klebsiella pneumoniae strain BD_DM_782

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GM011_RS12870
• Protein ID: WP_004179815.1
• Coordinates: 2663091..2664413 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GM011_RS12875
• Protein ID: WP_004176198.1
• Coordinates: 2664413..2664679 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM011_RS12855	2658147..2660648	+	2502	WP_020323802.1	fimbrial biogenesis outer membrane usher protein	-
GM011_RS12860	2660636..2661640	+	1005	WP_004179822.1	type 1 fimbrial protein	-
GM011_RS12865	2661782..2663074	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
GM011_RS12870	2663091..2664413	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
GM011_RS12875	2664413..2664679	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GM011_RS12880	2664945..2665271	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
GM011_RS12885	2665268..2665768	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GM011_RS12890	2665746..2667125	-	1380	WP_004179808.1	purine permease	-
GM011_RS12895	2667471..2668625	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
GM011_RS12900	2668606..2669529	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2462932..2699503	236571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T144140 WP_004179815.1 NZ_CP046381:c2664413-2663091 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T144140 NZ_CP061750:65030-65305 [Escherichia coli O15:H12]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTACTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTATGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT144140 WP_004176198.1 NZ_CP046381:c2664679-2664413 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT144140 NZ_CP061750:64746-65030 [Escherichia coli O15:H12]
ATGCAAATGAAAAACAATACCGCACAAACAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAATCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCAGACAGCCTCAATACTGACAATCCGTTACCGGTGCCTCGCTGA