Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 710007..710686 Replicon chromosome
Accession NZ_CP046381
Organism Klebsiella pneumoniae strain BD_DM_782

Toxin (Protein)


Gene name ykfI Uniprot ID A0A0C7K7A4
Locus tag GM011_RS03475 Protein ID WP_020324801.1
Coordinates 710345..710686 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0C7KEL2
Locus tag GM011_RS03470 Protein ID WP_020324792.1
Coordinates 710007..710324 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GM011_RS03445 705221..708373 + 3153 WP_022631414.1 AIDA repeat-containing protein -
GM011_RS03450 708466..708705 + 240 WP_020324820.1 DUF905 domain-containing protein -
GM011_RS03455 708808..709266 + 459 WP_020324813.1 antirestriction protein -
GM011_RS03460 709282..709758 + 477 WP_020324797.1 RadC family protein -
GM011_RS03465 709767..709994 + 228 WP_020324796.1 DUF987 domain-containing protein -
GM011_RS03470 710007..710324 + 318 WP_020324792.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GM011_RS03475 710345..710686 + 342 WP_020324801.1 TA system toxin CbtA family protein Toxin
GM011_RS03480 710802..711635 + 834 WP_020324805.1 DUF4942 domain-containing protein -
GM011_RS03490 711938..712444 + 507 WP_002917636.1 G/U mismatch-specific DNA glycosylase -
GM011_RS03495 712544..714385 - 1842 WP_004174339.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimA / fimI / fimC / fimD / fimD / fimF / fimG 662435..711635 49200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12818.91 Da        Isoelectric Point: 9.1484

>T144130 WP_020324801.1 NZ_CP046381:710345-710686 [Klebsiella pneumoniae]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDETVIQEHIDAGITLADAVNFLVEKYGLVRIDRNGF
VWQEQSPYLRAVDILRARQATGLLRQSRNNAIR

Download         Length: 342 bp

>T144130 NZ_CP061749:3746408-3746626 [Escherichia coli O15:H12]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11915.51 Da        Isoelectric Point: 6.6246

>AT144130 WP_020324792.1 NZ_CP046381:710007-710324 [Klebsiella pneumoniae]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASMTGKFSDAECRKLDETFPHFISQMESMLITGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPIQR

Download         Length: 318 bp

>AT144130 NZ_CP061749:3746008-3746382 [Escherichia coli O15:H12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7K7A4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C7KEL2

References