Detailed information of TA system TA144124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3838022..3839167
• Replicon: chromosome
• Accession: NZ_CP046378
• Organism: Shewanella algae strain RQs-106

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GMX02_RS16985
• Protein ID: WP_054744620.1
• Coordinates: 3838022..3838768 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7X5Z3H2
• Locus tag: GMX02_RS16990
• Protein ID: WP_025011540.1
• Coordinates: 3838805..3839167 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX02_RS16960	3833239..3833805	-	567	WP_025011535.1	hypoxanthine phosphoribosyltransferase	-
GMX02_RS16965	3834217..3834942	+	726	WP_156020808.1	basic amino acid ABC transporter substrate-binding protein	-
GMX02_RS16970	3834949..3835626	+	678	WP_025011537.1	amino acid ABC transporter permease	-
GMX02_RS16975	3835619..3836344	+	726	WP_025886969.1	amino acid ABC transporter ATP-binding protein	-
GMX02_RS16980	3836443..3838008	+	1566	WP_107353203.1	hypothetical protein	-
GMX02_RS16985	3838022..3838768	-	747	WP_054744620.1	RES family NAD+ phosphorylase	Toxin
GMX02_RS16990	3838805..3839167	-	363	WP_025011540.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
GMX02_RS16995	3839400..3840053	+	654	WP_025886967.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
GMX02_RS17000	3840184..3840825	+	642	WP_044735675.1	DnaJ domain-containing protein	-
GMX02_RS17005	3840966..3841703	+	738	WP_123115805.1	thioredoxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (55-205)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-118)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 28870.61 Da
• Isoelectric Point: 4.6112

>T144124 WP_054744620.1 NZ_CP046378:c3838768-3838022 [Shewanella algae]
MDFSALEPQDFFPRPYLGRGYRLVESQEEAATLSLVDDFDEQMLLESLLDEVKPPYRSGTEHLHYLLKTPFRYPPLRHGS
RFGSITMESFFYASESWQTCLAEVAYYRFVFMADMLEPYLEPLRSEHMLYSVDINARDCADLTLLQGQEVQQQLTHKSNY
TYTQAMGAWLLQQQAQMIRYTSARAEEGVNLALHDPLVLQSTEPQECEYWICQSSADKVAFSRRNSRSMPLTWFLKDFLQ
DGQLPRPA

Nucleotide

• Length: 747 bp

>T144124 NZ_CP061749:c2479575-2479381 [Escherichia coli O15:H12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13125.14 Da
• Isoelectric Point: 10.4675

>AT144124 WP_025011540.1 NZ_CP046378:c3839167-3838805 [Shewanella algae]
MNTLEKANAAEVLFKAFSNACSELQLTQAEASRIIGVNPSTLSRNSGKGFKPDSKQGELQLQLIRLYRSLYAIAGGEKAF
MRHWLNSPNKALNGTPKTLLQTVVGLVSVNQYLDAMRGKV

Nucleotide

• Length: 363 bp

>AT144124 NZ_CP061749:2479204-2479382 [Escherichia coli O15:H12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z3H2