Detailed information of TA system TA144123

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1421350..1421992
• Replicon: chromosome
• Accession: NZ_CP046378
• Organism: Shewanella algae strain RQs-106

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: GMX02_RS06280
• Protein ID: WP_045283532.1
• Coordinates: 1421570..1421992 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A7X5Z2N1
• Locus tag: GMX02_RS06275
• Protein ID: WP_028779782.1
• Coordinates: 1421350..1421589 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX02_RS06250	1417033..1417860	+	828	WP_044734948.1	prolipoprotein diacylglyceryl transferase	-
GMX02_RS06255	1417860..1418654	+	795	WP_044734947.1	thymidylate synthase	-
GMX02_RS06260	1418771..1419946	+	1176	WP_025010547.1	Na+/H+ antiporter NhaA	-
GMX02_RS06265	1419958..1420326	+	369	WP_096141176.1	hypothetical protein	-
GMX02_RS06270	1420343..1421251	+	909	WP_025010548.1	transcriptional activator NhaR	-
GMX02_RS06275	1421350..1421589	+	240	WP_028779782.1	succinate dehydrogenase assembly factor 2	Antitoxin
GMX02_RS06280	1421570..1421992	+	423	WP_045283532.1	hypothetical protein	Toxin
GMX02_RS06285	1422010..1423620	-	1611	WP_156019104.1	L-aspartate oxidase	-
GMX02_RS06290	1423820..1424398	+	579	WP_025010551.1	RNA polymerase sigma factor RpoE	-
GMX02_RS06295	1424423..1425043	+	621	WP_025887842.1	anti-sigma factor	-
GMX02_RS06300	1425053..1425985	+	933	WP_025887844.1	MucB/RseB C-terminal domain-containing protein	-
GMX02_RS06305	1425996..1426475	+	480	WP_028779785.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-135)

Antitoxin

• Flavinator of succinate dehydrogenase (5-75)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16302.81 Da
• Isoelectric Point: 7.6048

>T144123 WP_045283532.1 NZ_CP046378:1421570-1421992 [Shewanella algae]
VAGQRHRFQLSSSFSQYLSLTVLAAICLSSFLAWPALDYPFYLSLKYLCLLLMLAGFGLALWRLRRWQLCFWLNELGEGQ
FEPGSSFELSGRPWVSPFVVTFTYQGEDNYGRCWLFADMFDDTDYRHLCRLLLQRSKSAN

Nucleotide

• Length: 423 bp

>T144123 NZ_CP061749:2193991-2194094 [Escherichia coli O15:H12]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9247.74 Da
• Isoelectric Point: 5.0633

>AT144123 WP_028779782.1 NZ_CP046378:1421350-1421589 [Shewanella algae]
MSTPRVKWACRRGMLELDVLFQPFVEQHYETLSPEDKALFVRLLECEDPELFAWFMGHEQCPDPQLAQMVVRVRGRAAP

Nucleotide

• Length: 240 bp

>AT144123 NZ_CP061749:c2194132-2193861 [Escherichia coli O15:H12]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z2N1