Detailed information of TA system TA144122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4752939..4753767
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: GMX10_RS21755
• Protein ID: WP_161547114.1
• Coordinates: 4753228..4753767 (+)
• Length: 180 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: GMX10_RS21750
• Protein ID: WP_161547113.1
• Coordinates: 4752939..4753247 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS21720	4748102..4748299	+	198	WP_161547108.1	DUF2724 domain-containing protein	-
GMX10_RS21725	4748305..4748667	+	363	WP_161547109.1	DUF5347 domain-containing protein	-
GMX10_RS21730	4748738..4748971	+	234	WP_161547110.1	DUF2732 domain-containing protein	-
GMX10_RS21735	4748968..4749810	+	843	WP_161547111.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
GMX10_RS21740	4749917..4750096	+	180	WP_043881945.1	hypothetical protein	-
GMX10_RS21745	4750096..4752600	+	2505	WP_161547112.1	replication endonuclease	-
GMX10_RS21750	4752939..4753247	+	309	WP_161547113.1	DUF1778 domain-containing protein	Antitoxin
GMX10_RS21755	4753228..4753767	+	540	WP_161547114.1	GNAT family N-acetyltransferase	Toxin
GMX10_RS21760	4754180..4755247	-	1068	WP_015840841.1	phage portal protein	-
GMX10_RS21765	4755250..4756962	-	1713	WP_103164692.1	terminase family protein	-
GMX10_RS21770	4757109..4757954	+	846	WP_161547115.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4736254..4775044	38790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (62-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (18-96)

Sequences

Toxin

Protein

• Length: 180 a.a.
• Molecular weight: 19507.59 Da
• Isoelectric Point: 8.0336

>T144122 WP_161547114.1 NZ_CP046377:4753228-4753767 [Pectobacterium polaris]
MENPQNEVNNGLMVCSYQSDITYPGVKHFDCGNAVINSFVRNSLKKSVKDGNCAAKILIDKKTGELVGFCSFSAYSLEKG
KLATTIGGSLPNDVGVIRLNMLGVATKEQNKGRGQDLLCEFFEHVKLIHQALPIKGVYLDADPKAINFYARLGFVELKEP
PNSFGAVPMFLAIQHILAA

Nucleotide

• Length: 540 bp

>T144122 NZ_CP061749:c1950584-1950210 [Escherichia coli O15:H12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGTGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11287.97 Da
• Isoelectric Point: 10.5548

>AT144122 WP_161547113.1 NZ_CP046377:4752939-4753247 [Pectobacterium polaris]
MTNVAMFPTDAKSVKDARVELKTSSDLKNVLREAAVVAGLDLSAFILNAALERAEGVLENKRRRELSEHSWQQVNQLLSE
PAAPTLALQALMRRRKDGKSSK

Nucleotide

• Length: 309 bp

>AT144122 NZ_CP061749:c1951041-1950673 [Escherichia coli O15:H12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA