Detailed information of TA system TA144121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4657873..4658426
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GMX10_RS21255
• Protein ID: WP_039513711.1
• Coordinates: 4657873..4658187 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3I2T3
• Locus tag: GMX10_RS21260
• Protein ID: WP_014699357.1
• Coordinates: 4658190..4658426 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS21235	4653187..4654202	+	1016	Protein_4154	P-type DNA transfer ATPase VirB11	-
GMX10_RS21240	4654246..4654548	+	303	WP_161547089.1	conjugal transfer protein	-
GMX10_RS21245	4655342..4657078	+	1737	WP_039513716.1	type IV secretory system conjugative DNA transfer family protein	-
GMX10_RS21250	4657087..4657839	+	753	WP_039513714.1	hypothetical protein	-
GMX10_RS21255	4657873..4658187	-	315	WP_039513711.1	CcdB family protein	Toxin
GMX10_RS21260	4658190..4658426	-	237	WP_014699357.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GMX10_RS21265	4658748..4659692	-	945	WP_039513709.1	DUF1738 domain-containing protein	-
GMX10_RS21270	4660299..4663385	-	3087	WP_052237790.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4631164..4677992	46828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (4-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11489.41 Da
• Isoelectric Point: 7.9859

>T144121 WP_039513711.1 NZ_CP046377:c4658187-4657873 [Pectobacterium polaris]
MQSTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPERLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T144121 NZ_CP061749:1938688-1938942 [Escherichia coli O15:H12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9004.06 Da
• Isoelectric Point: 6.4610

>AT144121 WP_014699357.1 NZ_CP046377:c4658426-4658190 [Pectobacterium polaris]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRYHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT144121 NZ_CP061749:1938440-1938691 [Escherichia coli O15:H12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3I2T3