Detailed information of TA system TA144112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 1080171..1081010
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6P1S3C2
• Locus tag: GMX10_RS05075
• Protein ID: WP_039482128.1
• Coordinates: 1080171..1080533 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GMX10_RS05080
• Protein ID: WP_039482130.1
• Coordinates: 1080537..1081010 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS05055	1075520..1076273	-	754	Protein_986	type I-E CRISPR-associated protein Cas5/CasD	-
GMX10_RS05060	1076283..1077350	-	1068	WP_039482121.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
GMX10_RS05065	1077354..1078001	-	648	WP_039482124.1	type I-E CRISPR-associated protein Cse2/CasB	-
GMX10_RS05070	1077994..1079556	-	1563	WP_039482127.1	type I-E CRISPR-associated protein Cse1/CasA	-
GMX10_RS05075	1080171..1080533	+	363	WP_039482128.1	type II toxin-antitoxin system HigB family toxin	Toxin
GMX10_RS05080	1080537..1081010	+	474	WP_039482130.1	helix-turn-helix domain-containing protein	Antitoxin
GMX10_RS05085	1081000..1083717	-	2718	WP_052012030.1	CRISPR-associated helicase/endonuclease Cas3	-
GMX10_RS05090	1083749..1084301	-	553	Protein_993	polymer-forming cytoskeletal protein	-
GMX10_RS05095	1084471..1084659	-	189	WP_039482134.1	hypothetical protein	-
GMX10_RS05100	1084983..1085906	+	924	WP_039482136.1	aminoimidazole riboside kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1071378..1081010	9632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (108-154)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14134.36 Da
• Isoelectric Point: 9.7305

>T144112 WP_039482128.1 NZ_CP046377:1080171-1080533 [Pectobacterium polaris]
MRVISAKAITDAMKKHAKWKVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFALLENESRKGPLDIYVFDIN
GNSCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T144112 NZ_CP061749:c665868-665404 [Escherichia coli O15:H12]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGATGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17776.06 Da
• Isoelectric Point: 4.2547

>AT144112 WP_039482130.1 NZ_CP046377:1080537-1081010 [Pectobacterium polaris]
MRNTSLGSNSSVGISGLIENLPLLLQEASEQLNSLSVLLSACMEPIEDDNDLQERMECINQLYLYSSDVNHIPATFAELI
ADRVYEYEKKNRDVPHVSQAEALAFFIEERGLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPIETFID

Nucleotide

• Length: 474 bp

>AT144112 NZ_CP061749:c666203-665868 [Escherichia coli O15:H12]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A6P1S3C2