Detailed information of TA system TA144108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 615997..616547
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GMX10_RS02900
• Protein ID: WP_039513069.1
• Coordinates: 616239..616547 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: GMX10_RS02895
• Protein ID: WP_052237773.1
• Coordinates: 615997..616236 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS02870	611347..611658	-	312	WP_103160829.1	AzlD domain-containing protein	-
GMX10_RS02875	611655..612389	-	735	WP_103160830.1	AzlC family ABC transporter permease	-
GMX10_RS02880	612617..613453	-	837	WP_039513064.1	AraC family transcriptional regulator	-
GMX10_RS02885	613672..615291	-	1620	WP_039513065.1	Tar ligand binding domain-containing protein	-
GMX10_RS02890	615737..615922	-	186	WP_039513067.1	hypothetical protein	-
GMX10_RS02895	615997..616236	+	240	WP_052237773.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GMX10_RS02900	616239..616547	+	309	WP_039513069.1	CcdB family protein	Toxin
GMX10_RS02905	616690..616854	-	165	WP_039513071.1	glucose uptake inhibitor SgrT	-
GMX10_RS02910	616945..618603	+	1659	WP_161546649.1	HTH-type transcriptional regulator SgrR	-
GMX10_RS02915	618818..619801	+	984	WP_161547143.1	thiamine ABC transporter substrate binding subunit	-
GMX10_RS02920	619777..621384	+	1608	WP_039278253.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11766.60 Da
• Isoelectric Point: 9.3356

>T144108 WP_039513069.1 NZ_CP046377:616239-616547 [Pectobacterium polaris]
MQYKVYRNNGNSNSYPYLLNVQSDIIGELHTRLVIPLFPLNKVARPPARRLTPIVTVEGNDYLIMTHEMASVRRSQLGHE
VMDAQMYRKTIKDAVDFLLDGF

Nucleotide

• Length: 309 bp

>T144108 NZ_CP061745:37422-37925 [Enterobacter hormaechei]
ATGTTTTCAGCACAAGCCGTATACGATTTTTCGGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTGGTACAGCAACACAACGGGCGTATATTACGTGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCGGAT
GCCCCCAGCGTTACGCTTGGCCGTCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGGGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCAAATGCCAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAACAGAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9068.43 Da
• Isoelectric Point: 10.0137

>AT144108 WP_052237773.1 NZ_CP046377:615997-616236 [Pectobacterium polaris]
MPVTSIRHKKTVSVTLEPMLLQQAREAGINLSAILTEALKREIRLSEGEKWKQQNQKALQELNRITDKHGLLSDDDRTF

Nucleotide

• Length: 240 bp

>AT144108 NZ_CP061745:37128-37397 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA