Detailed information of TA system TA144103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2517953..2518587
• Replicon: chromosome
• Accession: NZ_CP046376
• Organism: Pectobacterium parmentieri strain HC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GMW39_RS11745
• Protein ID: WP_161545906.1
• Coordinates: 2518183..2518587 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GMW39_RS11740
• Protein ID: WP_161545905.1
• Coordinates: 2517953..2518183 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMW39_RS11730	2514437..2516329	-	1893	WP_161545904.1	hypothetical protein	-
GMW39_RS11735	2516326..2517693	-	1368	WP_040034770.1	hypothetical protein	-
GMW39_RS11740	2517953..2518183	+	231	WP_161545905.1	antitoxin	Antitoxin
GMW39_RS11745	2518183..2518587	+	405	WP_161545906.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GMW39_RS11750	2518972..2520186	+	1215	WP_161545907.1	tyrosine-type recombinase/integrase	-
GMW39_RS11755	2520485..2520721	-	237	WP_025919190.1	hypothetical protein	-
GMW39_RS24360	2521229..2521453	+	225	WP_198339761.1	hypothetical protein	-
GMW39_RS11765	2521530..2521728	+	199	Protein_2319	IS30 family transposase	-
GMW39_RS11770	2522076..2523371	-	1296	WP_161545908.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15074.30 Da
• Isoelectric Point: 8.0239

>T144103 WP_161545906.1 NZ_CP046376:2518183-2518587 [Pectobacterium parmentieri]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISAITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQTYGFDSAVHT
GQIRAELAKQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVNR

Nucleotide

• Length: 405 bp

>T144103 NZ_CP061744:c302339-302037 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8693.77 Da
• Isoelectric Point: 4.3951

>AT144103 WP_161545905.1 NZ_CP046376:2517953-2518183 [Pectobacterium parmentieri]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEQDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT144103 NZ_CP061744:c302030-301743 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTAGGTTAACAATCAAACCCTCTGGGGATTAA