Detailed information of TA system TA144099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 219137..220859
• Replicon: chromosome
• Accession: NZ_CP046376
• Organism: Pectobacterium parmentieri strain HC

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: K6YUL7
• Locus tag: GMW39_RS01090
• Protein ID: WP_008844785.1
• Coordinates: 219921..220859 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: GMW39_RS01085
• Protein ID: WP_000095577.1
• Coordinates: 219137..219928 (+)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMW39_RS01050	214307..214630	-	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GMW39_RS01055	214811..215092	+	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
GMW39_RS01060	215089..215715	+	627	WP_000667170.1	hypothetical protein	-
GMW39_RS01065	215699..216595	+	897	WP_161546444.1	type-F conjugative transfer system secretin TraK	-
GMW39_RS01070	216598..217887	+	1290	WP_161545417.1	TraB/VirB10 family protein	-
GMW39_RS01075	217962..218534	+	573	WP_161545418.1	type IV conjugative transfer system lipoprotein TraV	-
GMW39_RS01080	218531..218917	+	387	WP_161545419.1	hypothetical protein	-
GMW39_RS01085	219137..219928	+	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GMW39_RS01090	219921..220859	+	939	WP_008844785.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GMW39_RS01095	220991..221683	+	693	WP_001228924.1	DsbC family protein	-
GMW39_RS01100	221683..224082	+	2400	WP_161545420.1	type IV secretion system protein TraC	-
GMW39_RS01105	224075..224422	+	348	WP_011788500.1	hypothetical protein	-
GMW39_RS01110	224406..224918	+	513	WP_193021830.1	S26 family signal peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(3'')-Ia	-	160473..274251	113778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-272)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35528.79 Da
• Isoelectric Point: 4.9912

>T144099 WP_008844785.1 NZ_CP046376:219921-220859 [Pectobacterium parmentieri]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMAVQESVEYEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGVSREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLTSLQK
HFTERDYAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T144099 NZ_CP061723:c5117460-5117173 [Pseudomonas putida]
ATGACCTATAGCCTCGACTTCGATGCCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACCGTGCGTCAGCAGTTCAA
GAAGAAACTGGCAGAGGTCCTGCTGAACCCTCGAATCGAGGCAAACCGCCTTCACTCGCTGCCGGATTGCTACAAGATAA
AGCTTCGCAGCAGCGGGTTTAGGCTGGTCTATCAGGTCATTGACCAAGAGGTCGTGGTTTTTGTCGTCGCTGTCGATCGC
CGTGAAAGAGACCAAGCTTACAAAAAGGCTGCTGAGCGACTTGAGTAG

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT144099 WP_000095577.1 NZ_CP046376:219137-219928 [Pectobacterium parmentieri]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT144099 NZ_CP061723:c5117689-5117450 [Pseudomonas putida]
ATGGCTTCCATCAACATCCGCGTCGACGATGATCTCAAAGCCCGCGCCTACAAAGAGCTCGAGCGCCTCGGTGTAACCCC
CTCCGAACTCATGCGGCAGGCTCTGCAATATGTCGCTGAGCGGGGCAAGCTACCCTTCAGGCCAGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCTACCGTACAAGAGCGGCTTGCTTCTGCGCAGCGCGTGAGGGTCCAGCTGGATGACCTATAG

Structures

Toxin

• AlphaFold DB: K6YUL7