Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2048969..2049460 Replicon chromosome
Accession NZ_CP046363
Organism Macrococcus canis strain Epi0082

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag GL258_RS10940 Protein ID WP_138072443.1
Coordinates 2048969..2049319 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GL258_RS10945 Protein ID WP_138072445.1
Coordinates 2049320..2049460 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GL258_RS10920 2046289..2047056 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
GL258_RS10925 2047061..2047504 - 444 WP_138072439.1 anti-sigma B factor RsbW -
GL258_RS10930 2047509..2047832 - 324 WP_086043353.1 anti-sigma factor antagonist -
GL258_RS10935 2047913..2048920 - 1008 WP_138072441.1 PP2C family protein-serine/threonine phosphatase -
GL258_RS10940 2048969..2049319 - 351 WP_138072443.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GL258_RS10945 2049320..2049460 - 141 WP_138072445.1 antitoxin MazE Antitoxin
GL258_RS10950 2049522..2050664 - 1143 WP_138072447.1 alanine racemase -
GL258_RS10955 2050690..2051046 - 357 WP_164953884.1 holo-ACP synthase -
GL258_RS10960 2050988..2051509 - 522 WP_188019435.1 PH domain-containing protein -
GL258_RS10965 2051493..2053004 - 1512 WP_138072453.1 PH domain-containing protein -
GL258_RS10970 2052994..2053470 - 477 WP_138072455.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12750.96 Da        Isoelectric Point: 9.7528

>T144097 WP_138072443.1 NZ_CP046363:c2049319-2048969 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTAYHLEKDSVILLEQIRT
IDKYRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T144097 NZ_CP061723:4948256-4948558 [Pseudomonas putida]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGACCGGACTACGGGACTTAAGCGCCAGAGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCGGGCGACGTATCTCCGGTAGGCCATGGCGTGAGTGAGCTCAAAATTCACT
ATGGTCCCGGCTATCGGGTGTACTTCCATCAGACGGGCAGCACCTTCGTCATTCTGCTCTGTGGCGGCGACAAAAGCAGT
CAAAAGCGAGACATCAAGGTTGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT144097 WP_138072445.1 NZ_CP046363:c2049460-2049320 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTIDGE

Download         Length: 141 bp

>AT144097 NZ_CP061723:4948551-4948844 [Pseudomonas putida]
ATGACTGAAACCTTCTCTGAATTCGATCCTGCCGCGTACCTCACTACACCTGAAGCAATCGCGCAGTTCATGACCGATGC
ACTTGAAACCGGCGATGCCAGCTATGTCGCTAAAGCTGTCGGCGTGGTTGCACGTGCCAAGGGCATGAGCGAACTGGCGA
AAGAGACGGGGCTGTCCCGAGAGCAGCTTTACAGGTCATTCAGCGATCATGGCAACCCCACGCTGAAGTCGTTTCTGACC
GTGATGAAGGCATTGGGAATAGACATGACGGCGCGGCCTCACCTCGCTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References