Detailed information of TA system TA144096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1237716..1238203
• Replicon: chromosome
• Accession: NZ_CP046363
• Organism: Macrococcus canis strain Epi0082

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GL258_RS06460
• Protein ID: WP_138071166.1
• Coordinates: 1237937..1238203 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GL258_RS06455
• Protein ID: WP_138071164.1
• Coordinates: 1237716..1237940 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GL258_RS06420	1233274..1233720	-	447	WP_188020190.1	GNAT family N-acetyltransferase	-
GL258_RS06425	1233803..1234543	+	741	WP_188019009.1	hypothetical protein	-
GL258_RS06430	1234553..1235443	+	891	WP_188019010.1	phosphotransferase	-
GL258_RS06435	1235629..1236114	+	486	WP_188019011.1	NUDIX domain-containing protein	-
GL258_RS06440	1236120..1236887	+	768	WP_138071158.1	hypothetical protein	-
GL258_RS06445	1236905..1237177	-	273	WP_138071160.1	Txe/YoeB family addiction module toxin	-
GL258_RS06450	1237170..1237427	-	258	WP_138071162.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GL258_RS06455	1237716..1237940	+	225	WP_138071164.1	toxin-antitoxin system, antitoxin component	Antitoxin
GL258_RS06460	1237937..1238203	+	267	WP_138071166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GL258_RS06465	1238291..1238800	+	510	WP_138071168.1	hypothetical protein	-
GL258_RS06470	1238961..1240178	+	1218	WP_138071170.1	ATP-binding protein	-
GL258_RS06475	1240314..1241576	+	1263	WP_086042501.1	Y-family DNA polymerase	-
GL258_RS06480	1241723..1241917	+	195	WP_188019012.1	hypothetical protein	-
GL258_RS06485	1242076..1242375	+	300	WP_096076536.1	DUF4298 domain-containing protein	-
GL258_RS06490	1242439..1242918	-	480	WP_164954063.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10539.32 Da
• Isoelectric Point: 10.4082

>T144096 WP_138071166.1 NZ_CP046363:1237937-1238203 [Macrococcus canis]
MKYSIEYDPKVIKQLRKMDPQSSRLLLQWINKNIVHCEDPRSSGKALTGNYSGFWRYRVGQFRIITKIEDDKLIILVISI
DHRKNIYR

Nucleotide

• Length: 267 bp

>T144096 NZ_CP061723:c4593235-4592939 [Pseudomonas putida]
ATGGAAAAGAGAACGCCTCACTGTCCGTTGGAAAGGGTAAAAGTCTTGGCTGCTGCAAGGCGCATCAGCCCTACAGGCGC
TGCACTGAGAGGCGCCAGAGCCCTGGGAATGGATTATCCGGGCATGCTTGAAGTGATCTCCTGTTTGGAGCGTACTGATT
TCTACAAGAGCATGACCAGCCATATGGATCACCGCGTTTGGCAGGACGTCTATCGTCCGCTTACAGCATTTGGGTACGTT
TATCTGAAAGTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8745.95 Da
• Isoelectric Point: 4.5816

>AT144096 WP_138071164.1 NZ_CP046363:1237716-1237940 [Macrococcus canis]
MATITIRVSEDEKNFLKYMSEFMNLSLTDLIKNYTIEDLEDLFDTHVADKAYIEWSKHKQTVSHNDVMKEFGLL

Nucleotide

• Length: 225 bp

>AT144096 NZ_CP061723:c4592936-4592535 [Pseudomonas putida]
ATGAGATGTCCAGTATGCGGCGGTGCGGAGCTTGCACCTGATGTTCAGGGGATGCCCTATAGCTATAAAGGAGAGGCGAC
CGTCATCCCTGACGTAAGTGGCGATTACTGTTGCGCCTGTGGTGAATGTGTACTTAGCCACGATGAAGCCATGCGTGTCA
GTAACCTGATGACGGCATTCGAGCGTCAAGTCAACGCAGCTGTTGTGGATCCTTCCTTCATTGCTTCAATCCGCCGTAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATTTTCGGGGGTGGCGTCAACGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACACCACCCGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTCGAGGAAGTGCGTACTGCCT
GA