Detailed information of TA system TA144090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1021071..1022308
• Replicon: chromosome
• Accession: NZ_CP046359
• Organism: Streptococcus pneumoniae strain 573

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: GL188_RS05220
• Protein ID: WP_000405365.1
• Coordinates: 1021547..1022308 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GL188_RS05215
• Protein ID: WP_000579599.1
• Coordinates: 1021071..1021547 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GL188_RS05195	1016144..1016998	+	855	WP_024477726.1	ADP-ribosylglycohydrolase family protein	-
GL188_RS05200	1017110..1017994	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
GL188_RS05205	1018157..1019896	+	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
GL188_RS05210	1020171..1020626	+	456	WP_000500465.1	hypothetical protein	-
GL188_RS05215	1021071..1021547	+	477	WP_000579599.1	helix-turn-helix domain-containing protein	Antitoxin
GL188_RS05220	1021547..1022308	+	762	WP_000405365.1	zeta toxin family protein	Toxin
GL188_RS05225	1022336..1023492	+	1157	Protein_1029	hypothetical protein	-
GL188_RS05230	1023566..1024822	-	1257	WP_197097166.1	ISL3 family transposase	-
GL188_RS05235	1025732..1026091	+	360	WP_000003745.1	hypothetical protein	-
GL188_RS05240	1026101..1026466	+	366	WP_000263051.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M) / erm(B)	-	916770..1025196	108426
	-	flank	IS/Tn	-	-	1023566..1024822	1256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T144090 WP_000405365.1 NZ_CP046359:1021547-1022308 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T144090 NZ_CP061717:c11531-11187 [Pediococcus acidilactici]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATTTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGCCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGACTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT144090 WP_000579599.1 NZ_CP046359:1021071-1021547 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144090 NZ_CP061717:c11788-11525 [Pediococcus acidilactici]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA