Detailed information of TA system TA144089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 988112..989355
• Replicon: chromosome
• Accession: NZ_CP046359
• Organism: Streptococcus pneumoniae strain 573

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: GL188_RS05050
• Protein ID: WP_024477678.1
• Coordinates: 988588..989355 (+)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GL188_RS05045
• Protein ID: WP_000579597.1
• Coordinates: 988112..988588 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GL188_RS05015	984009..984962	-	954	WP_001155339.1	IS30-like element ISSpn8 family transposase	-
GL188_RS05020	985334..985972	+	639	WP_024477682.1	hypothetical protein	-
GL188_RS05025	985982..987067	+	1086	WP_024477681.1	toprim domain-containing protein	-
GL188_RS05030	987118..987351	+	234	WP_024477680.1	hypothetical protein	-
GL188_RS05035	987348..987731	+	384	WP_002933294.1	hypothetical protein	-
GL188_RS05040	987769..988041	+	273	WP_024477679.1	hypothetical protein	-
GL188_RS05045	988112..988588	+	477	WP_000579597.1	helix-turn-helix domain-containing protein	Antitoxin
GL188_RS05050	988588..989355	+	768	WP_024477678.1	zeta toxin family protein	Toxin
GL188_RS05055	989390..991009	+	1620	WP_078133416.1	DUF4297 domain-containing protein	-
GL188_RS05060	990996..991535	+	540	WP_024477676.1	hypothetical protein	-
GL188_RS05065	991525..994164	+	2640	WP_078133417.1	SMC family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M) / erm(B)	-	916770..1025196	108426
	-	flank	IS/Tn	-	-	984009..984962	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 29310.39 Da
• Isoelectric Point: 7.4386

>T144089 WP_024477678.1 NZ_CP046359:988588-989355 [Streptococcus pneumoniae]
MGIQDYTDSEFKQALERNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFARKMVESLVTELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEKLAIFERIQIYQRDRSCVYDSKENTTSAAEVLQECLFGKWSKVEEEML
KVGEEILETLCNRTE

Nucleotide

• Length: 768 bp

>T144089 NZ_CP061714:c1210589-1209297 [Luteimonas sp. MC1825]
GTGACCGACGCCACCGTCCGCCTCTGGGGCCGCGACATCGGCGCGGTGAGCTGGCTGGAAGACCGCGACCGCGCGGTGTT
CCAGTACATGCCGGACTTCGCCGACAGCGGCATCCGCGTCGCCCCGATCATGATGCCGCCAGGCCGCGAACCCTTCGAAT
TCCCCGGCCTCCCGCGCGAGGCGTTCAAGGGCCTGCCGGGCATGCTCGCCGACGCCCTGCCCGACCGCTTCGGCAACGCG
CTCATCGACGCGTGGCTCGCCGCGCAAGGCCGCGCGCCCGGCAGCTTCAACCCGGTCGAGCGCCTTTGCTACATCGGCAG
CCGCGGCATGGGCGCGCTGGAATTCCATCCCAGCCTGTCGGGCCTGCCGCGGCAGTCACGCGCCGTCGAGATCGATGCCC
TCACCCGGCTCGCCAACGACGTGCTCAACCAGCGCGGCCAGCTTGCCGGCGCGCTGATGGGCGATGACGACCGCGGCGAC
CTCGAGGACATCCTGCGCGTGGGCACCTCCGCCGGTGGCGCGCGCGCCAAGGCCGTGCTGGCCTGGAACGAACAGACCGG
CGAGTTCCGCTCCGGCCAGGTCAAGGCCGGCGAAGGCTTCAGCGCCTGGCTGATCAAGTTCGACGGCATCAGCGACAACC
GCGACAAGGAGCTCGCCGACCCGCAAGGTTTCGGCCTGGTGGAATACGCCTTCCACCTGCTGGCGGTCGCCGCCGGCATC
GACATGAGCGCCTGCCGCATCCACCGCGAAGGCGGCCGCGCGCATTTCATGACCCGCCGCTTCGACCGCACATCCAGCGG
CGGCAAGCTGCACATGCAGTCACTGGCCGCGCTGCGCCATTTCGATTTCAACGCCGCCGGCGCGCATTCCTACGAGCAGG
CCGTGGAAACCATCCGCCTGCTCGGCCTGCCTGCGCTGGACATCGAGCAGCAGTTCCGCCGCGCCGTGTTCAACGTGCTG
ATCCGCAACCAGGACGACCACGTCAAGAACATCGCCTTCCTGATGAACCGCAAGGGCGAGTGGCGCCTGTCGCCCGCGTT
CGATGTCAGCTACGCGTACAACCCGCAGGGCAGCTGGACGCACCAGCACCAGATGAGCCTCAACGGCAAGCGCGACCATT
TTGAGGCGGAGGACTTCGTGCGGTTCGGGGCGTTCTGCGGCATCAAGCCGACGAAGGCGCGCGAGCTGGTGGCGGAGATC
CGAACGCAGGTGGAAGGTTGGATGGGGTTTGCCGAGCGGGCGGGCGTGCCGGAGCGCAGGGCGGTGGAGATCGGGCGGGC
CATGCGGGCGTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18235.60 Da
• Isoelectric Point: 4.3571

>AT144089 WP_000579597.1 NZ_CP046359:988112-988588 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144089 NZ_CP061714:c1210945-1210586 [Luteimonas sp. MC1825]
ATGGACTCTTACACAGACCGTCATGCTACGATTCAAGCCTCCCTCCTCGCCCTCGGCGAGCGTCTGGCCCAGCTGCGCCT
CGCCCGCAACCTGACTCAGGCCGCGCTGGCCCGCGAGGCCGGCACGTCCGCCAGCAGCATCAAGCGCCTGGAAGCCGGCG
CCAACGCCTCGCTCGACACCCTGCTGCGCGTGCTGCAGGTCCTGGGGCTGGATGGCCGCCTGCTCGACAGCCTGCCCGCC
CCCGGCGTGCGCCCCGTGGAGCGCGCCAGGCTCGGCGGCCGCGAGCGCCGCCGCGCCAGCACGCCTGCGGAAACACCCAA
GGCCACCGACTGGGCCTGGGGCGAGGACGACAGTCCGTGA