Detailed information of TA system TA144086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1016552..1017789
• Replicon: chromosome
• Accession: NZ_CP046358
• Organism: Streptococcus pneumoniae strain 566

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: GL186_RS05200
• Protein ID: WP_000405365.1
• Coordinates: 1017028..1017789 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GL186_RS05195
• Protein ID: WP_000579599.1
• Coordinates: 1016552..1017028 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GL186_RS05175	1011625..1012479	+	855	WP_024477726.1	ADP-ribosylglycohydrolase family protein	-
GL186_RS05180	1012591..1013475	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
GL186_RS05185	1013638..1015377	+	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
GL186_RS05190	1015652..1016107	+	456	WP_000500465.1	hypothetical protein	-
GL186_RS05195	1016552..1017028	+	477	WP_000579599.1	helix-turn-helix domain-containing protein	Antitoxin
GL186_RS05200	1017028..1017789	+	762	WP_000405365.1	zeta toxin family protein	Toxin
GL186_RS05205	1017817..1018973	+	1157	Protein_1026	hypothetical protein	-
GL186_RS05210	1019047..1020303	-	1257	WP_197097166.1	ISL3 family transposase	-
GL186_RS05215	1021213..1021572	+	360	WP_000003745.1	hypothetical protein	-
GL186_RS05220	1021582..1021947	+	366	WP_000263051.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M) / erm(B)	-	910828..1020677	109849
	-	flank	IS/Tn	-	-	1019047..1020303	1256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T144086 WP_000405365.1 NZ_CP046358:1017028-1017789 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T144086 NZ_CP061714:65050-65346 [Luteimonas sp. MC1825]
ATGAAGAACATCGCCGCTTTTGATGCCGCCGACTATCTCGACAACGACATTGTCATTGCTGAGTACCTGAACGCTGCGTT
GGAAGATGAGAATCCCAACGTCTTTCTTCAGGCAATCGCTGACGTTGCAAAGGCTCGAGGTATGAGCAAGCTGGCCAAGG
ACACCGGACTTGGTCGTGAGAGCCTGTACAAGGCGTTGGCCCCGGGCGCAAAGCCACGCTACGACACTGTACTTAAGCTG
GTTCGTGCGCTTGGCGTTGAGCTGCATACAACGCCAGCTCATGCAAGCCAGGCCTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT144086 WP_000579599.1 NZ_CP046358:1016552-1017028 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144086 NZ_CP061714:64751-65047 [Luteimonas sp. MC1825]
ATGAGCATATTCCTTCGCACGTCGGAGTTCGACGCGTGGCTGAAAGGGCTACGGGATCCGATAGCAACAGCTCGCATCGC
GGCGCGCATCCGCTCCGCAGAGGCGGGCAACTTCGGTGACTGTGCCCCGGTGGGTGACGGCATCTCCGAGATGCGGATTC
ACGTCGGCCCTGGATATCGTTTGTACTACTGCCGCCGCGGCGACGTCACCTATTTGCTGCTCTGCGCGGGAGACAAGTCG
TCCCAGCCGAAGGACATCCTGAAAGCCAAGACTCTCCTCAAGAGTCTGGAGAACTGA