Detailed information of TA system TA144075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1147994..1149231
• Replicon: chromosome
• Accession: NZ_CP046355
• Organism: Streptococcus pneumoniae strain 475

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: GL183_RS05960
• Protein ID: WP_000405365.1
• Coordinates: 1147994..1148755 (-)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GL183_RS05965
• Protein ID: WP_000579599.1
• Coordinates: 1148755..1149231 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GL183_RS05940	1143405..1145270	-	1866	WP_000261795.1	relaxase/mobilization nuclease domain-containing protein	-
GL183_RS05945	1145257..1145622	-	366	WP_000263051.1	MobC family plasmid mobilization relaxosome protein	-
GL183_RS05950	1145632..1145991	-	360	WP_000003745.1	hypothetical protein	-
GL183_RS05955	1146810..1147966	-	1157	Protein_1128	hypothetical protein	-
GL183_RS05960	1147994..1148755	-	762	WP_000405365.1	zeta toxin family protein	Toxin
GL183_RS05965	1148755..1149231	-	477	WP_000579599.1	helix-turn-helix domain-containing protein	Antitoxin
GL183_RS05970	1149676..1150131	-	456	WP_000500465.1	hypothetical protein	-
GL183_RS05975	1150406..1152145	-	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
GL183_RS05980	1152308..1153192	-	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
GL183_RS05985	1153304..1154158	-	855	WP_024477726.1	ADP-ribosylglycohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M)	-	1124423..1252390	127967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T144075 WP_000405365.1 NZ_CP046355:c1148755-1147994 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T144075 NZ_CP061705:466233-466622 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTACTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT144075 WP_000579599.1 NZ_CP046355:c1149231-1148755 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144075 NZ_CP061705:465969-466226 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGAGCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA