Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 458325..458993 Replicon chromosome
Accession NZ_CP046355
Organism Streptococcus pneumoniae strain 475

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B7M7X4
Locus tag GL183_RS02490 Protein ID WP_001132284.1
Coordinates 458325..458504 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag GL183_RS02495 Protein ID WP_000961810.1
Coordinates 458541..458993 (+) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GL183_RS02465 454299..455618 + 1320 WP_024477878.1 glycoside hydrolase family 32 protein -
GL183_RS02475 456166..457437 - 1272 WP_001113213.1 replication-associated recombination protein A -
GL183_RS02480 457735..457950 + 216 WP_075329187.1 hypothetical protein -
GL183_RS02485 457940..458188 + 249 WP_000797240.1 hypothetical protein -
GL183_RS02490 458325..458504 + 180 WP_001132284.1 type II toxin-antitoxin system HicA family toxin Toxin
GL183_RS02495 458541..458993 + 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GL183_RS02500 459284..459754 + 471 WP_000257084.1 DUF3013 family protein -
GL183_RS02505 459773..460861 + 1089 WP_024477877.1 M50 family metallopeptidase -
GL183_RS02510 460842..461270 + 429 WP_024477876.1 NUDIX hydrolase -
GL183_RS02515 461267..461740 + 474 WP_000631889.1 GNAT family N-acetyltransferase -
GL183_RS02520 461878..462828 + 951 WP_024477875.1 50S ribosomal protein L11 methyltransferase -
GL183_RS02525 462830..463573 + 744 WP_001188201.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T144073 WP_001132284.1 NZ_CP046355:458325-458504 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T144073 NZ_CP061704:481767-482117 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT144073 WP_000961810.1 NZ_CP046355:458541-458993 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT144073 NZ_CP061704:481480-481761 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7M7X4

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References