Detailed information of TA system TA144072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1134245..1135488
• Replicon: chromosome
• Accession: NZ_CP046354
• Organism: Streptococcus pneumoniae strain 310

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: GLO74_RS05845
• Protein ID: WP_024477678.1
• Coordinates: 1134245..1135012 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GLO74_RS05850
• Protein ID: WP_000579597.1
• Coordinates: 1135012..1135488 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLO74_RS05830	1129436..1132075	-	2640	WP_078133417.1	SMC family ATPase	-
GLO74_RS05835	1132065..1132604	-	540	WP_024477676.1	hypothetical protein	-
GLO74_RS05840	1132591..1134210	-	1620	WP_078133416.1	DUF4297 domain-containing protein	-
GLO74_RS05845	1134245..1135012	-	768	WP_024477678.1	zeta toxin family protein	Toxin
GLO74_RS05850	1135012..1135488	-	477	WP_000579597.1	helix-turn-helix domain-containing protein	Antitoxin
GLO74_RS05855	1135559..1135831	-	273	WP_024477679.1	hypothetical protein	-
GLO74_RS05860	1135869..1136252	-	384	WP_002933294.1	hypothetical protein	-
GLO74_RS05865	1136249..1136482	-	234	WP_024477680.1	hypothetical protein	-
GLO74_RS05870	1136533..1137618	-	1086	WP_024477681.1	toprim domain-containing protein	-
GLO74_RS05875	1137628..1138266	-	639	WP_024477682.1	hypothetical protein	-
GLO74_RS05880	1138638..1139591	+	954	WP_001155339.1	IS30-like element ISSpn8 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M)	-	1076300..1205679	129379
	-	flank	IS/Tn	-	-	1138638..1139591	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 29310.39 Da
• Isoelectric Point: 7.4386

>T144072 WP_024477678.1 NZ_CP046354:c1135012-1134245 [Streptococcus pneumoniae]
MGIQDYTDSEFKQALERNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFARKMVESLVTELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEKLAIFERIQIYQRDRSCVYDSKENTTSAAEVLQECLFGKWSKVEEEML
KVGEEILETLCNRTE

Nucleotide

• Length: 768 bp

>T144072 NZ_CP061703:601029-601331 [Mesoflavibacter profundi]
ATGAGCAAAAACAAATATCGCATAAGTCAACAAGCGATTGAGGATTTAGATAAAATTTGGATTTATACGCTCAATAAATG
GTCTAAAGAACAAGCTGACAGATATTACGACTTGATAATTACGGAAATTGAATTTATAGCTGACAACTTTATGACTGGAA
AGTCAGCGGAACAAACTCGAAAGAATTATCGAGTGACTAAAATAAAATCCCATTTAATTTTTTATAGAAAAGTGGAAAAT
GATATCGTGGAAATTGTTAGAATTTTACACCAACGAATGGATATTAAAAAACGACTGAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18235.60 Da
• Isoelectric Point: 4.3571

>AT144072 WP_000579597.1 NZ_CP046354:c1135488-1135012 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144072 NZ_CP061703:600790-601032 [Mesoflavibacter profundi]
ATGGGAAAAAACACATCAATATCACTCGGAAATCATTTTGAGGATTTTATAAGAGAAGAAGTAAATTCTGGAAGATATGG
TTCGGTTAGCGAAGTAATTCGTTCTGCTTTACGTTTGTTAGAACGTGAAGAAAAAAAGGAAAGGGAACTGATTAAAGCTC
TCGAGGTTGGGGAAAATAGTGGATTTGTTGAAAATTTTGACCCAAAACAACATCTGAAAGAATTACATCGTAAACACTTA
TGA