Detailed information of TA system TA144057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1892507..1894145
• Replicon: chromosome
• Accession: NZ_CP046348
• Organism: Citrobacter portucalensis strain FDAARGOS_738

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FOC46_RS09495
• Protein ID: WP_032934262.1
• Coordinates: 1892507..1893835 (-)
• Length: 443 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FOC46_RS09500
• Protein ID: WP_072025544.1
• Coordinates: 1893840..1894145 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC46_RS09475	1888485..1889969	+	1485	WP_003830130.1	chromosome partitioning protein ParB	-
FOC46_RS09480	1889953..1890492	+	540	WP_003830133.1	DUF2857 domain-containing protein	-
FOC46_RS09485	1890510..1891643	+	1134	WP_003830134.1	helix-turn-helix domain-containing protein	-
FOC46_RS09490	1891709..1892380	-	672	WP_000863683.1	hypothetical protein	-
FOC46_RS09495	1892507..1893835	-	1329	WP_032934262.1	type II toxin-antitoxin system HipA family toxin	Toxin
FOC46_RS09500	1893840..1894145	-	306	WP_072025544.1	helix-turn-helix transcriptional regulator	Antitoxin
FOC46_RS09505	1894771..1898013	+	3243	WP_003830139.1	ATP-binding protein	-
FOC46_RS09510	1898328..1899026	+	699	WP_003830141.1	TIGR03761 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1885753..1963301	77548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 443 a.a.
• Molecular weight: 50601.29 Da
• Isoelectric Point: 5.1237

>T144057 WP_032934262.1 NZ_CP046348:c1893835-1892507 [Citrobacter portucalensis]
MRRTQQRLAIWMNGIRVGFWEKARGEDLLQYLPEWVVDEQGRPLSLSLPFTPGNQLWRGNVVRDYFDNLLPDSEGIRRRL
AVRYQSESLEPFDLLAELGRDCVGAIQLLNVDEEPTDLFSVNYRPLSEADIATTLRNTTAASLPGRQDETDDLRLSIAGA
QEKTALLWHEEQWCLPEGNTPTTHIFKLPLGLVGNMQADMSTSVENEWLCSLLLEHYGLPVAKTQIAQFEDQKALVVERF
DRRWSGDRQWIIRLPQEDMCQALGVSPLRKYQSDGGPGISDIMDILSHSDQAEQDKEQFFRAQIIFWLLAATDGHAKNFS
IAIEPQSHYHLTPLYDVLSAWPVIGHGNNQISWQRCKLAMAVRGSSNHYHIYRIQRRHWINHGELTGLGRRQVESMMDDI
ISSTPEVIERVSALLPESFPPELAECIFEGMRQQCRRLAGRE

Nucleotide

• Length: 1329 bp

>T144057 NZ_CP061700:352405-352752 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTATGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11377.05 Da
• Isoelectric Point: 10.1481

>AT144057 WP_072025544.1 NZ_CP046348:c1894145-1893840 [Citrobacter portucalensis]
MNTVYPLKTLNQIRPLLIGFRKANGLTQKEVSERLGVTQQTYARLEANPASASMERLFRVFTVLGVEMVLSSGPQILSSM
SSSTMEESEQQKDSPARREKW

Nucleotide

• Length: 306 bp

>AT144057 NZ_CP061700:352752-353051 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA