Detailed information of TA system TA144056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1833659..1834235
• Replicon: chromosome
• Accession: NZ_CP046348
• Organism: Citrobacter portucalensis strain FDAARGOS_738

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FOC46_RS09215
• Protein ID: WP_003830043.1
• Coordinates: 1833948..1834235 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: FOC46_RS09210
• Protein ID: WP_003830041.1
• Coordinates: 1833659..1833961 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC46_RS09190	1829295..1829837	+	543	WP_003830032.1	hypothetical protein	-
FOC46_RS09195	1830126..1832276	+	2151	WP_003830033.1	pyruvate/proton symporter BtsT	-
FOC46_RS09200	1832383..1832577	+	195	WP_003830038.1	YbdD/YjiX family protein	-
FOC46_RS09205	1832590..1833546	+	957	WP_003830040.1	GTPase	-
FOC46_RS09210	1833659..1833961	-	303	WP_003830041.1	BrnA antitoxin family protein	Antitoxin
FOC46_RS09215	1833948..1834235	-	288	WP_003830043.1	BrnT family toxin	Toxin
FOC46_RS09220	1834387..1835451	-	1065	WP_003830044.1	DUF2955 domain-containing protein	-
FOC46_RS09225	1835441..1836508	-	1068	WP_003830045.1	HlyD family secretion protein	-
FOC46_RS09230	1836514..1836978	-	465	WP_003830047.1	MarR family transcriptional regulator	-
FOC46_RS09235	1837127..1837291	-	165	WP_003019180.1	DUF1127 domain-containing protein	-
FOC46_RS09240	1837469..1838881	+	1413	WP_003830051.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11235.60 Da
• Isoelectric Point: 7.5237

>T144056 WP_003830043.1 NZ_CP046348:c1834235-1833948 [Citrobacter portucalensis]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T144056 NZ_CP061700:326434-326802 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11495.05 Da
• Isoelectric Point: 9.5650

>AT144056 WP_003830041.1 NZ_CP046348:c1833961-1833659 [Citrobacter portucalensis]
MSMVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDVPVTEDEQWSEAHRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT144056 NZ_CP061700:326216-326437 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA