Detailed information of TA system TA144050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 569595..570179
• Replicon: chromosome
• Accession: NZ_CP046346
• Organism: Burkholderia multivorans strain FDAARGOS_722

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FOC30_RS16625
• Protein ID: WP_035950265.1
• Coordinates: 569595..569891 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: FOC30_RS16630
• Protein ID: WP_006409266.1
• Coordinates: 569895..570179 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC30_RS16605	565107..566207	-	1101	WP_006413771.1	chorismate synthase	-
FOC30_RS16610	566376..568310	-	1935	WP_006413765.1	MFS transporter	-
FOC30_RS16615	568458..568913	-	456	WP_006402096.1	CBS domain-containing protein	-
FOC30_RS16620	569074..569391	+	318	WP_006402097.1	DUF962 domain-containing protein	-
FOC30_RS16625	569595..569891	+	297	WP_035950265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOC30_RS16630	569895..570179	+	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
FOC30_RS16635	570263..570451	+	189	WP_155822971.1	hypothetical protein	-
FOC30_RS16640	570618..571946	-	1329	WP_039224705.1	YihY family inner membrane protein	-
FOC30_RS16645	572091..572702	+	612	WP_155822153.1	NAD(P)H:quinone oxidoreductase	-
FOC30_RS16650	572699..573115	+	417	WP_155822154.1	DUF2069 domain-containing protein	-
FOC30_RS16655	573134..574555	+	1422	WP_155822155.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11271.01 Da
• Isoelectric Point: 9.7646

>T144050 WP_035950265.1 NZ_CP046346:569595-569891 [Burkholderia multivorans]
MVTLLRSTEFNDWLANLRDQRGKARIAARLISAQLGNFGEYRVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKS
TQRKDIKRAAQMARELKE

Nucleotide

• Length: 297 bp

>T144050 NZ_CP061699:1303221-1303499 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGGCTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCAGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCTCCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAACGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT144050 WP_006409266.1 NZ_CP046346:569895-570179 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT144050 NZ_CP061699:1303511-1303816 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: J4SGI3