Detailed information of TA system TA144024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 646962..648208
• Replicon: chromosome
• Accession: NZ_CP046335
• Organism: Streptococcus mitis strain FDAARGOS_684

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A380KWA4
• Locus tag: FOB92_RS03325
• Protein ID: WP_000405376.1
• Coordinates: 647438..648208 (+)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: FOB92_RS03320
• Protein ID: WP_000579609.1
• Coordinates: 646962..647438 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB92_RS03300	641977..643725	+	1749	WP_001254284.1	ABC transporter ATP-binding protein/permease	-
FOB92_RS03305	643983..645242	+	1260	WP_000870440.1	TRZ/ATZ family protein	-
FOB92_RS03310	645493..645993	+	501	WP_001287267.1	50S ribosomal protein L10	-
FOB92_RS03315	646066..646434	+	369	WP_001196965.1	50S ribosomal protein L7/L12	-
FOB92_RS03320	646962..647438	+	477	WP_000579609.1	helix-turn-helix domain-containing protein	Antitoxin
FOB92_RS03325	647438..648208	+	771	WP_000405376.1	zeta toxin family protein	Toxin
FOB92_RS03335	649865..650296	+	432	WP_025169729.1	hypothetical protein	-
FOB92_RS03340	650299..650748	+	450	WP_000370556.1	hypothetical protein	-
FOB92_RS03345	650851..651321	+	471	WP_000447038.1	winged helix DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	623350..678495	55145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29394.27 Da
• Isoelectric Point: 7.2202

>T144024 WP_000405376.1 NZ_CP046335:647438-648208 [Streptococcus mitis]
VEIQDYTDSEFKHALERNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAKLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQSEELAIFERIQIYQRDRSCVYDSGEDKTSAATVLHDLLFGEWNQVEKEML
KSGEERLKDLTNRNDC

Nucleotide

• Length: 771 bp

>T144024 NZ_CP061636:17925-18281 [Acinetobacter seifertii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGACCTACGTGTTCAGCATCGAGGTAAATCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGTGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18227.67 Da
• Isoelectric Point: 4.3457

>AT144024 WP_000579609.1 NZ_CP046335:646962-647438 [Streptococcus mitis]
MIGKNIKSLRKTHDLTQPEFAQIIGISRNSLSRYENGTSPVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144024 NZ_CP061636:18274-18576 [Acinetobacter seifertii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A380KWA4