Detailed information of TA system TA144019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 264166..264779
• Replicon: chromosome
• Accession: NZ_CP046331
• Organism: Cupriavidus metallidurans strain FDAARGOS_675

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q1LPG3
• Locus tag: FOB83_RS02075
• Protein ID: WP_011515865.1
• Coordinates: 264166..264345 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FOB83_RS02080
• Protein ID: WP_029310017.1
• Coordinates: 264375..264779 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB83_RS02050	259563..260063	+	501	WP_011515870.1	peptidyl-prolyl cis-trans isomerase	-
FOB83_RS02055	260132..260923	+	792	WP_011515869.1	UDP-2,3-diacylglucosamine diphosphatase	-
FOB83_RS02060	260972..261715	-	744	WP_011515868.1	serine O-acetyltransferase	-
FOB83_RS02065	262044..262913	-	870	WP_011515867.1	RNA methyltransferase	-
FOB83_RS02070	263141..263962	+	822	WP_011515866.1	inositol monophosphatase	-
FOB83_RS02075	264166..264345	+	180	WP_011515865.1	type II toxin-antitoxin system HicA family toxin	Toxin
FOB83_RS02080	264375..264779	+	405	WP_029310017.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FOB83_RS02085	265106..267712	+	2607	WP_035821829.1	DNA mismatch repair protein MutS	-
FOB83_RS02090	267709..268761	+	1053	WP_011515862.1	hypothetical protein	-
FOB83_RS02095	268856..269389	-	534	WP_029308195.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6790.07 Da
• Isoelectric Point: 12.3707

>T144019 WP_011515865.1 NZ_CP046331:264166-264345 [Cupriavidus metallidurans]
MHSSTLIRLLEQKGWKLVRTHGSHFTFKHPRHMLIVTVPHPKKDLPTGTVRNILRTAGL

Nucleotide

• Length: 180 bp

>T144019 NZ_CP061628:2286-2573 [Acinetobacter seifertii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGAAGATCCGCTGAGGATCTCAATCCAGGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAGAGGCGTACTAATGATGTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAGGCGGAGCGAGATAAATATGAGAACGGTTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14618.79 Da
• Isoelectric Point: 4.9285

>AT144019 WP_029310017.1 NZ_CP046331:264375-264779 [Cupriavidus metallidurans]
MDFPIAIHKDANSVYGVTVPDVAGCYSAGESIEEAVRNAREAITTHVVALLSLGEEVVLKTTPIGTLMAQPEYADAIWAL
ADIDMSKLDARPERINISLPRFVLHKIDMFVERRHETRSGFLARVALDAIAGSV

Nucleotide

• Length: 405 bp

>AT144019 NZ_CP061628:2560-2874 [Acinetobacter seifertii]
ATGAGAACGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAAGGGACGGGTCCTGATGATCACCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATCCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTCTGCGTGAAGCAATGTTAGAAGATTTAAAAAATCATCGATAA

Structures

Toxin

• AlphaFold DB: A0A2L0WZI7