Detailed information of TA system TA144015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1941607..1943408
• Replicon: chromosome
• Accession: NZ_CP046329
• Organism: Shewanella putrefaciens strain FDAARGOS_681

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FOB89_RS08685
• Protein ID: WP_011919798.1
• Coordinates: 1941607..1942953 (+)
• Length: 449 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FOB89_RS08690
• Protein ID: WP_011919797.1
• Coordinates: 1942950..1943408 (+)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB89_RS08660	1937047..1938633	+	1587	WP_011919802.1	VWA domain-containing protein	-
FOB89_RS08665	1938644..1939177	+	534	WP_011919801.1	hypothetical protein	-
FOB89_RS08670	1939302..1940219	+	918	WP_011919800.1	IS5-like element ISSpu14 family transposase	-
FOB89_RS08675	1940248..1940610	-	363	WP_011919799.1	hypothetical protein	-
FOB89_RS08680	1940644..1941039	-	396	WP_041420585.1	hypothetical protein	-
FOB89_RS08685	1941607..1942953	+	1347	WP_011919798.1	type II toxin-antitoxin system HipA family toxin	Toxin
FOB89_RS08690	1942950..1943408	+	459	WP_011919797.1	helix-turn-helix domain-containing protein	Antitoxin
FOB89_RS08695	1943592..1944248	-	657	WP_011919796.1	EcsC family protein	-
FOB89_RS08700	1944412..1944834	+	423	WP_011919795.1	helix-turn-helix domain-containing protein	-
FOB89_RS08705	1944897..1948034	-	3138	WP_011919794.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1939302..1940219	917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-409)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-139)

Sequences

Toxin

Protein

• Length: 449 a.a.
• Molecular weight: 50997.60 Da
• Isoelectric Point: 4.6127

>T144015 WP_011919798.1 NZ_CP046329:1941607-1942953 [Shewanella putrefaciens]
MEQLTIQAYINGQWSDIAQLSFPESEQGNYRLTSLDYEQTYALAELDKDDLHAVSLNHPVSLFFDDLGRPGWLRFLDDIM
PSGASRRYWLNYLDIADLTLDEQNFVLLKFGTIAPVGNLRVKESLPKRTAIADTLKFTVDDVRNRAADFLDYAQHQGAAA
GGATGAGGEAPKLLLRCDDEQNIWIDSYQDEPQNRDLHYLVKYPRGARLPIDCDILRAEYHYYHELAEMGFETIPTANMR
LEEGHSYPSLWLPRFDIYFNELGQQRYAMESVYSILGKGAGVTLDHEQTIRALIDKITHSHMVLEQGYQFDVRAFVIEWV
RRDLLNILFGNSDNHGRNTAFLRGDGWIRLAPIYDFAPMKADPEGIARTTKWEAPLEIGGEYDFQSIAEALADLVPAQEL
LAELKRTAQQCLGLEQRLIGRGVPQRIIDMPAIGFAYLEDKLTRWGLV

Nucleotide

• Length: 1347 bp

>T144015 NZ_CP061623:c8095-7736 [Acinetobacter seifertii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 16944.30 Da
• Isoelectric Point: 10.6618

>AT144015 WP_011919797.1 NZ_CP046329:1942950-1943408 [Shewanella putrefaciens]
MSDKQTTTETAKDTIARLRANRQQQSDIAIEASQHRQPVNIGEAQRKQDAKRASKQARGVSTVERKNVIYSTAQKLLHGE
LTQGQALKALRVEVLGLTQDEYANLVKVSRKTLSDVENDRGSYKTDILNRLFKPFGLKVGIVPISTGVTWLL

Nucleotide

• Length: 459 bp

>AT144015 NZ_CP061623:c7736-7437 [Acinetobacter seifertii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA