Detailed information of TA system TA144003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 12145..13063
• Replicon: plasmid unnamed1
• Accession: NZ_CP046318
• Organism: Campylobacter coli strain FDAARGOS_735

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: FOC43_RS08660
• Protein ID: WP_002779836.1
• Coordinates: 12145..12828 (-)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: FOC43_RS08665
• Protein ID: WP_002779838.1
• Coordinates: 12821..13063 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC43_RS08625	7199..7522	-	324	WP_002790618.1	hypothetical protein	-
FOC43_RS08630	7712..8080	+	369	WP_002779826.1	hypothetical protein	-
FOC43_RS08635	8058..10214	+	2157	WP_002779827.1	relaxase/mobilization nuclease domain-containing protein	-
FOC43_RS08640	10253..10477	-	225	WP_002779829.1	hypothetical protein	-
FOC43_RS08645	10480..11166	-	687	WP_002779831.1	AAA family ATPase	-
FOC43_RS08650	11214..11609	-	396	WP_002779833.1	conjugal transfer protein TraM	-
FOC43_RS08655	11673..12140	-	468	WP_002779834.1	signal peptidase I	-
FOC43_RS08660	12145..12828	-	684	WP_002779836.1	Fic family protein	Toxin
FOC43_RS08665	12821..13063	-	243	WP_002779838.1	hypothetical protein	Antitoxin
FOC43_RS08670	13307..13774	+	468	WP_072214055.1	IS200/IS605 family transposase	-
FOC43_RS08675	13776..15026	+	1251	WP_032685593.1	transposase	-
FOC43_RS08680	15077..15400	-	324	WP_002790618.1	hypothetical protein	-
FOC43_RS08685	15590..15958	+	369	WP_002779826.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38104	38104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 25919.05 Da
• Isoelectric Point: 10.1133

>T144003 WP_002779836.1 NZ_CP046318:c12828-12145 [Campylobacter coli]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKKERIITLQAAKKLELNPIKGNFDYQHLKDIHKALFKDVYTWA
GKDRMEVGLHGNFGKYAPNGAIINFVPGKELDKYSKIIFDELAKNNYLKNSKDLNHFAKNLAKFMGEINALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLISKHKMIHACVEASQLKPGRLEALIKDNLKNFRQNLDLEQNKGMSL

Nucleotide

• Length: 684 bp

>T144003 NZ_CP061584:c87903-87547 [Acinetobacter seifertii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGACCTACGTGTTCAGCATCGAGGTAAATCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGTGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9478.72 Da
• Isoelectric Point: 4.2508

>AT144003 WP_002779838.1 NZ_CP046318:c13063-12821 [Campylobacter coli]
MQKQYPEVYSLEESLAILKKYKDDLTKEQYEQNKSIICGFAIENMFANEEDIINLIKVDKQEKTPDEIIAEYKKEWGVND

Nucleotide

• Length: 243 bp

>AT144003 NZ_CP061584:c87554-87252 [Acinetobacter seifertii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA