Detailed information of TA system TA143997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4092001..4092656
• Replicon: chromosome
• Accession: NZ_CP046309
• Organism: Mycobacterium tuberculosis strain FDAARGOS_750

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOC58_RS19280
• Protein ID: WP_031646990.1
• Coordinates: 4092001..4092402 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TIK2
• Locus tag: FOC58_RS19285
• Protein ID: WP_003407272.1
• Coordinates: 4092399..4092656 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC58_RS19265	4087473..4088858	-	1386	WP_003407260.1	cytochrome P450	-
FOC58_RS19270	4088936..4089970	+	1035	WP_003407264.1	AraC family transcriptional regulator	-
FOC58_RS19275	4090016..4091746	-	1731	WP_009940170.1	PE family protein	-
FOC58_RS19280	4092001..4092402	-	402	WP_031646990.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC58_RS19285	4092399..4092656	-	258	WP_003407272.1	ribbon-helix-helix domain-containing protein	Antitoxin
FOC58_RS19290	4092739..4093698	-	960	WP_003407276.1	alpha/beta hydrolase	-
FOC58_RS19295	4093723..4094685	-	963	WP_003407279.1	alpha/beta hydrolase	-
FOC58_RS19300	4094684..4095421	+	738	WP_031647000.1	lysoplasmalogenase	-
FOC58_RS19305	4095502..4097469	+	1968	WP_003407285.1	primosomal protein N'	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14974.48 Da
• Isoelectric Point: 11.6897

>T143997 WP_031646990.1 NZ_CP046309:c4092402-4092001 [Mycobacterium tuberculosis]
MILVHSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Nucleotide

• Length: 402 bp

>T143997 NZ_CP061562:c24231-23875 [Acinetobacter seifertii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9443.58 Da
• Isoelectric Point: 6.4631

>AT143997 WP_003407272.1 NZ_CP046309:c4092656-4092399 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Nucleotide

• Length: 258 bp

>AT143997 NZ_CP061562:c23882-23580 [Acinetobacter seifertii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGGAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Antitoxin

• AlphaFold DB: G0TIK2