Detailed information of TA system TA143989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3529472..3530091
• Replicon: chromosome
• Accession: NZ_CP046309
• Organism: Mycobacterium tuberculosis strain FDAARGOS_750

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FOC58_RS16700
• Protein ID: WP_003404726.1
• Coordinates: 3529657..3530091 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: FOC58_RS16695
• Protein ID: WP_003898641.1
• Coordinates: 3529472..3529651 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC58_RS16685	3524945..3526525	+	1581	WP_003909313.1	serine hydrolase	-
FOC58_RS16690	3526522..3528915	+	2394	WP_016719446.1	cation-translocating P-type ATPase	-
FOC58_RS21030	3529188..3529346	+	159	WP_003404720.1	hypothetical protein	-
FOC58_RS16695	3529472..3529651	+	180	WP_003898641.1	antitoxin	Antitoxin
FOC58_RS16700	3529657..3530091	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FOC58_RS16705	3530189..3530962	+	774	WP_003404735.1	VOC family protein	-
FOC58_RS16710	3531027..3531476	+	450	WP_003404738.1	hypothetical protein	-
FOC58_RS16715	3531611..3531841	-	231	WP_003898642.1	hypothetical protein	-
FOC58_RS16720	3532008..3533516	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FOC58_RS16725	3533518..3534756	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T143989 WP_003404726.1 NZ_CP046309:3529657-3530091 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T143989 NZ_CP061531:32584-33105 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAACGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT143989 WP_003898641.1 NZ_CP046309:3529472-3529651 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT143989 NZ_CP061531:32307-32576 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5