Detailed information of TA system TA143985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3267091..3267724
• Replicon: chromosome
• Accession: NZ_CP046309
• Organism: Mycobacterium tuberculosis strain FDAARGOS_750

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOC58_RS15340
• Protein ID: WP_015629650.1
• Coordinates: 3267091..3267474 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FOC58_RS15345
• Protein ID: WP_003403368.1
• Coordinates: 3267569..3267724 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC58_RS15315	3262383..3262919	+	537	WP_003403341.1	50S ribosomal protein L10	-
FOC58_RS15320	3262956..3263348	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FOC58_RS15325	3263341..3264036	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FOC58_RS15330	3264107..3265612	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FOC58_RS15335	3265693..3266703	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FOC58_RS15340	3267091..3267474	-	384	WP_015629650.1	hypothetical protein	Toxin
FOC58_RS15345	3267569..3267724	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC58_RS15350	3267800..3268516	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FOC58_RS15355	3268792..3269100	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FOC58_RS15360	3269087..3269332	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FOC58_RS15365	3269442..3269879	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FOC58_RS15370	3269876..3270130	-	255	WP_003911263.1	antitoxin VapB7	-
FOC58_RS15375	3270244..3272607	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13886.68 Da
• Isoelectric Point: 6.0803

>T143985 WP_015629650.1 NZ_CP046309:c3267474-3267091 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRLIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T143985 NZ_CP061530:c3740290-3739994 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT143985 WP_003403368.1 NZ_CP046309:c3267724-3267569 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT143985 NZ_CP061530:c3739992-3739597 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8