Detailed information of TA system TA143977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 3153801..3154477
• Replicon: chromosome
• Accession: NZ_CP046309
• Organism: Mycobacterium tuberculosis strain FDAARGOS_750

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FOC58_RS14750
• Protein ID: WP_003898500.1
• Coordinates: 3153801..3154214 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FOC58_RS14755
• Protein ID: WP_003402915.1
• Coordinates: 3154211..3154477 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC58_RS14720	3149146..3149448	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FOC58_RS14725	3149508..3149786	-	279	WP_003402901.1	hypothetical protein	-
FOC58_RS14730	3149783..3151036	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
FOC58_RS14735	3151156..3151542	-	387	WP_003402907.1	VOC family protein	-
FOC58_RS14740	3151605..3152489	-	885	WP_003402909.1	SDR family oxidoreductase	-
FOC58_RS14745	3152585..3153487	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FOC58_RS14750	3153801..3154214	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
FOC58_RS14755	3154211..3154477	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
FOC58_RS14760	3154669..3156384	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FOC58_RS14765	3156462..3158066	+	1605	WP_031647091.1	amidohydrolase	-
FOC58_RS14770	3158063..3159043	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T143977 WP_003898500.1 NZ_CP046309:c3154214-3153801 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T143977 NZ_CP061530:2025516-2025692 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT143977 WP_003402915.1 NZ_CP046309:c3154477-3154211 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT143977 NZ_CP061530:2025738-2026154 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0