Detailed information of TA system TA143966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1272208..1272895
• Replicon: chromosome
• Accession: NZ_CP046309
• Organism: Mycobacterium tuberculosis strain FDAARGOS_750

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: FOC58_RS06270
• Protein ID: WP_003414624.1
• Coordinates: 1272452..1272895 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOC58_RS06265
• Protein ID: WP_031647372.1
• Coordinates: 1272208..1272465 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC58_RS06245	1267528..1268382	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FOC58_RS06250	1268438..1269601	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FOC58_RS06255	1269618..1270832	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FOC58_RS06260	1270840..1272081	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FOC58_RS06265	1272208..1272465	+	258	WP_031647372.1	ribbon-helix-helix domain-containing protein	Antitoxin
FOC58_RS06270	1272452..1272895	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC58_RS06275	1272975..1273637	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
FOC58_RS06280	1273733..1273924	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FOC58_RS06285	1274256..1276004	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
FOC58_RS06290	1276100..1276681	+	582	WP_003414644.1	fasciclin domain-containing protein	-
FOC58_RS06295	1276781..1277047	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T143966 WP_003414624.1 NZ_CP046309:1272452-1272895 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T143966 NZ_CP061527:c3545486-3545172 [Shigella dysenteriae]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTCCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8960.17 Da
• Isoelectric Point: 10.2193

>AT143966 WP_031647372.1 NZ_CP046309:1272208-1272465 [Mycobacterium tuberculosis]
VRTTIRIDDELYCEVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT143966 NZ_CP061527:c3545722-3545489 [Shigella dysenteriae]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Structures

Toxin

• PDB: 7VWO