Detailed information of TA system TA143964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 1225744..1226348
• Replicon: chromosome
• Accession: NZ_CP046309
• Organism: Mycobacterium tuberculosis strain FDAARGOS_750

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FOC58_RS06035
• Protein ID: WP_003414492.1
• Coordinates: 1225744..1226136 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FOC58_RS06040
• Protein ID: WP_003414495.1
• Coordinates: 1226133..1226348 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC58_RS06005	1220894..1221682	-	789	WP_192939560.1	CRISPR-associated endoribonuclease Cas6	-
FOC58_RS06010	1222016..1222561	-	546	WP_014584866.1	DUF1802 family protein	-
FOC58_RS06015	1222833..1223717	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FOC58_RS06020	1223720..1224607	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FOC58_RS06025	1224912..1225457	-	546	WP_014584866.1	DUF1802 family protein	-
FOC58_RS06030	1225454..1225723	-	270	WP_003414489.1	DUF2277 family protein	-
FOC58_RS06035	1225744..1226136	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC58_RS06040	1226133..1226348	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FOC58_RS06045	1226395..1227144	+	750	WP_003414497.1	enoyl-CoA hydratase	-
FOC58_RS06050	1227223..1228305	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
FOC58_RS06055	1228298..1229608	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FOC58_RS06060	1229611..1230438	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FOC58_RS06065	1230435..1231346	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1207273..1227601	20328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T143964 WP_003414492.1 NZ_CP046309:c1226136-1225744 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T143964 NZ_CP061527:c3160650-3160432 [Shigella dysenteriae]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT143964 WP_003414495.1 NZ_CP046309:c1226348-1226133 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT143964 NZ_CP061527:c3161050-3160676 [Shigella dysenteriae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8