Detailed information of TA system TA143922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 3732582..3733258
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FOC59_RS17315
• Protein ID: WP_003898500.1
• Coordinates: 3732582..3732995 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FOC59_RS17320
• Protein ID: WP_003402915.1
• Coordinates: 3732992..3733258 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS17285	3727927..3728229	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FOC59_RS17290	3728289..3728567	-	279	WP_003402901.1	hypothetical protein	-
FOC59_RS17295	3728564..3729817	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
FOC59_RS17300	3729937..3730323	-	387	WP_003402907.1	VOC family protein	-
FOC59_RS17305	3730386..3731270	-	885	WP_003903053.1	SDR family oxidoreductase	-
FOC59_RS17310	3731366..3732268	-	903	WP_031654206.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FOC59_RS17315	3732582..3732995	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
FOC59_RS17320	3732992..3733258	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
FOC59_RS17325	3733450..3735165	-	1716	WP_003903055.1	fatty-acid--CoA ligase FadD8	-
FOC59_RS17330	3735243..3736847	+	1605	WP_003903056.1	amidohydrolase	-
FOC59_RS17335	3736844..3737824	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T143922 WP_003898500.1 NZ_CP046308:c3732995-3732582 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T143922 NZ_CP061472:2670416-2670766 [Geobacillus thermoleovorans]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCGGAGCAGGGCGGCGTGCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGTTTTAGCCCGACGGTGATTGTCGCGGCGATTACGGCGCAAATCCAAAAAG
CGAAGCTGCCGACGCATGTCGAGATTGACGCGAAACGCTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATT
CGCACGATCGACAAGCAACGGCTGACCGATAAAATCACTCATTTGGACGATGAAATGATGGATAAAGTCGATGAGGCGCT
GCAAATTAGCTTAGGGCTGATCGACTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT143922 WP_003402915.1 NZ_CP046308:c3733258-3732992 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT143922 NZ_CP061472:2670130-2670411 [Geobacillus thermoleovorans]
GTGTCGGAATCTGGCGCAACAGCGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGTGCTCGTAAA
GCAGGAAAACGGCAACCGCAATGAACTCATTTATCAAGCGACGAAAATGTACATTCGCGAGCGGAAGAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCGAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0