Detailed information of TA system TA143914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 2449444..2449973
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: FOC59_RS11480
• Protein ID: WP_003417760.1
• Coordinates: 2449716..2449973 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FOC59_RS11475
• Protein ID: WP_003908225.1
• Coordinates: 2449444..2449719 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS21020	2446242..2447679	-	1438	Protein_2241	FAD-binding oxidoreductase	-
FOC59_RS11460	2447782..2448171	+	390	WP_003417745.1	DUF732 domain-containing protein	-
FOC59_RS11465	2448185..2448478	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
FOC59_RS11470	2448475..2449320	-	846	WP_003905054.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
FOC59_RS11475	2449444..2449719	+	276	WP_003908225.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
FOC59_RS11480	2449716..2449973	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
FOC59_RS11485	2450015..2451205	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
FOC59_RS11490	2451322..2451690	+	369	WP_003417765.1	FHA domain-containing protein	-
FOC59_RS11495	2451687..2452238	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
FOC59_RS11500	2452245..2452826	-	582	WP_003417769.1	ATP/GTP-binding protein	-
FOC59_RS11505	2452807..2453175	-	369	WP_003417772.1	DUF742 domain-containing protein	-
FOC59_RS11510	2453153..2453545	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T143914 WP_003417760.1 NZ_CP046308:2449716-2449973 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T143914 NZ_CP061470:c113292-112873 [Geobacillus zalihae]
ATGAAACATGATGTGATTTTGAATAAGGTCAGCATCATTGAACGATGTCTGAAACGAATTGACGAAGAATATGACCACGA
CCCCCAAAATTTACAAAATTATACAAAACAAGACTCGATCATTCTCAATTTACAGCGGGCGTGCGAGGCATGCATCGATT
TGGCCATGCATATCGTGGCCGAGCAGAAATTCGGACTGCCGCAGCATAGCCGCGATGCGTTCGCTCTCCTCGAAGAACAT
GGGGTCATCTCCCCTTCCGTAAGCAAAAAGATGAAGGCGATGGTCGGATTCCGCAACATCGCCGTTCACAACTACCAACA
ACTGAACCTTGGCATTTTGCAAGCCATCATCGACCACCATCTCGATGATTTTAAACACTTTGCCAAAGCCATCCTCGATT
ATGCCAAGAAAAACGGCTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10221.38 Da
• Isoelectric Point: 4.6652

>AT143914 WP_003908225.1 NZ_CP046308:2449444-2449719 [Mycobacterium tuberculosis]
MNISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT143914 NZ_CP061470:c113689-113282 [Geobacillus zalihae]
TTGCCAAACGAAATGGAGACGATCATCATTCAGACGCTCCGCCCGGCTCTTCACCCGTTCGTCATCTACCTGTTCGGTTC
AGCCGCCCGTGGGGCGATGCGCTCGGACAGTGATGTCGATATCGCCTTTGTCAGCGACGGCGAACCGCATGATCCGTATG
AGCTGTTTCGGCTCGCGGGGCATTTGGCTGACAAGTTAGGGCGAGATGTCGATCTTGTCGATTTGCGCCAAGCCAGCACC
GTGTTTCAAGCGCAAGTCGTCTCGAGCGGAAAGGTGATCGATTGTTGCGACGAACGGAAACGAATGGAGTTTGAAATGAA
AACGTTGAAAATGTATGCAAAGCTGAATGAAGAGAGGGCGCCAGTATTAAAACGAATTGCAGAAAGTGGGTCGGTGTATG
AAACATGA

Structures

Toxin

• PDB: 3OEI