Detailed information of TA system TA143909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 1810702..1811306
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FOC59_RS08635
• Protein ID: WP_003414492.1
• Coordinates: 1810702..1811094 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FOC59_RS08640
• Protein ID: WP_003414495.1
• Coordinates: 1811091..1811306 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS08605	1805852..1806640	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FOC59_RS08610	1806974..1807519	-	546	WP_003904931.1	DUF1802 family protein	-
FOC59_RS08615	1807791..1808675	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FOC59_RS08620	1808678..1809565	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FOC59_RS08625	1809870..1810415	-	546	WP_003908074.1	DUF1802 family protein	-
FOC59_RS08630	1810412..1810681	-	270	WP_003414489.1	DUF2277 family protein	-
FOC59_RS08635	1810702..1811094	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC59_RS08640	1811091..1811306	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FOC59_RS08645	1811353..1812102	+	750	WP_003908075.1	enoyl-CoA hydratase	-
FOC59_RS08650	1812181..1813263	-	1083	WP_003908076.1	ABC transporter ATP-binding protein	-
FOC59_RS08655	1813256..1814566	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FOC59_RS08660	1814569..1815396	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FOC59_RS08665	1815393..1816304	-	912	WP_003904073.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T143909 WP_003414492.1 NZ_CP046308:c1811094-1810702 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T143909 NZ_CP061404:c5358207-5357824 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT143909 WP_003414495.1 NZ_CP046308:c1811306-1811091 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT143909 NZ_CP061404:c5358449-5358207 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8