Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1783715..1784285 Replicon chromosome
Accession NZ_CP046308
Organism Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FOC59_RS08490 Protein ID WP_003414166.1
Coordinates 1783715..1784071 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FOC59_RS08495 Protein ID WP_003901465.1
Coordinates 1784055..1784285 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC59_RS08470 1779167..1780855 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FOC59_RS08475 1780859..1781185 - 327 WP_003414157.1 hypothetical protein -
FOC59_RS08480 1781358..1781945 + 588 WP_003914429.1 DUF3558 family protein -
FOC59_RS08485 1781964..1783613 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FOC59_RS08490 1783715..1784071 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FOC59_RS08495 1784055..1784285 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FOC59_RS08500 1784328..1785371 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FOC59_RS08505 1785562..1785837 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FOC59_RS08510 1786013..1786267 - 255 WP_003917684.1 hypothetical protein -
FOC59_RS08515 1786415..1786819 + 405 WP_003414181.1 hypothetical protein -
FOC59_RS08520 1786816..1787007 + 192 WP_003414184.1 hypothetical protein -
FOC59_RS08525 1787071..1788360 + 1290 Protein_1671 transposase family protein -
FOC59_RS08530 1788594..1788851 + 258 WP_003899489.1 hypothetical protein -
FOC59_RS08535 1788956..1789267 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T143907 WP_003414166.1 NZ_CP046308:c1784071-1783715 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T143907 NZ_CP061404:c4848116-4847832 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT143907 WP_003901465.1 NZ_CP046308:c1784285-1784055 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT143907 NZ_CP061404:c4848348-4848106 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References