Detailed information of TA system TA143905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1743717..1744396
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FOC59_RS08265
• Protein ID: WP_003414059.1
• Coordinates: 1743717..1744133 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FOC59_RS08270
• Protein ID: WP_003414061.1
• Coordinates: 1744130..1744396 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS08245	1739769..1740671	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FOC59_RS08250	1740740..1741492	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FOC59_RS08255	1741736..1742011	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FOC59_RS08260	1742008..1743630	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FOC59_RS08265	1743717..1744133	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FOC59_RS08270	1744130..1744396	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC59_RS08275	1744422..1744817	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FOC59_RS08280	1744814..1745083	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FOC59_RS08285	1745093..1746187	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FOC59_RS08290	1746184..1746603	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FOC59_RS08295	1746677..1747156	-	480	WP_003414073.1	dihydrofolate reductase	-
FOC59_RS08300	1747227..1748027	-	801	WP_003911953.1	thymidylate synthase	-
FOC59_RS08305	1748183..1748920	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T143905 WP_003414059.1 NZ_CP046308:c1744133-1743717 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T143905 NZ_CP061404:4176536-4176754 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT143905 WP_003414061.1 NZ_CP046308:c1744396-1744130 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT143905 NZ_CP061404:4176135-4176509 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64