Detailed information of TA system TA143899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1529559..1530199
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FOC59_RS07075
• Protein ID: WP_003412970.1
• Coordinates: 1529559..1529978 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FOC59_RS07080
• Protein ID: WP_003412975.1
• Coordinates: 1529975..1530199 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS07050	1525144..1525866	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FOC59_RS07055	1526383..1526610	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
FOC59_RS07060	1526607..1527008	+	402	WP_003412963.1	PIN domain-containing protein	-
FOC59_RS07065	1527043..1527963	-	921	WP_003412965.1	restriction endonuclease	-
FOC59_RS20955	1528304..1528549	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FOC59_RS07070	1528608..1529558	+	951	WP_003911916.1	Lsr2 family protein	-
FOC59_RS07075	1529559..1529978	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC59_RS07080	1529975..1530199	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FOC59_RS07085	1530230..1533023	-	2794	Protein_1388	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FOC59_RS07090	1533095..1533496	-	402	WP_003412981.1	hypothetical protein	-
FOC59_RS07095	1533496..1533966	-	471	WP_003412985.1	transcription antitermination factor NusB	-
FOC59_RS07100	1533969..1534532	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T143899 WP_003412970.1 NZ_CP046308:c1529978-1529559 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T143899 NZ_CP061401:46938-47354 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT143899 WP_003412975.1 NZ_CP046308:c1530199-1529975 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT143899 NZ_CP061401:46711-46941 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09