Detailed information of TA system TA143893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 928705..929331
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FOC59_RS04270
• Protein ID: WP_003410075.1
• Coordinates: 928933..929331 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FOC59_RS04265
• Protein ID: WP_003911750.1
• Coordinates: 928705..928932 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS04255	926744..927088	-	345	WP_003410065.1	ferredoxin family protein	-
FOC59_RS04260	927277..928446	-	1170	WP_003899126.1	ATP-binding protein	-
FOC59_RS04265	928705..928932	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC59_RS04270	928933..929331	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FOC59_RS04275	929514..929945	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FOC59_RS04280	930046..930480	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FOC59_RS04285	930917..931096	-	180	Protein_845	hypothetical protein	-
FOC59_RS04290	931157..931804	+	648	WP_071854215.1	transposase	-
FOC59_RS04295	931758..932348	+	591	WP_003907840.1	IS110 family transposase	-
FOC59_RS04300	932476..932850	-	375	Protein_848	HNH endonuclease	-
FOC59_RS04305	932901..934162	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T143893 WP_003410075.1 NZ_CP046308:928933-929331 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T143893 NZ_CP061399:4209192-4209410 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT143893 WP_003911750.1 NZ_CP046308:928705-928932 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT143893 NZ_CP061399:4208791-4209165 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8