Detailed information of TA system TA143891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 903399..904297
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: FOC59_RS04150
• Protein ID: WP_003410001.1
• Coordinates: 903399..903959 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: FOC59_RS04155
• Protein ID: WP_003410003.1
• Coordinates: 903956..904297 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS04120	898568..899221	-	654	WP_003409976.1	cutinase Cfp21	-
FOC59_RS04125	899336..899566	+	231	WP_003409980.1	DUF167 domain-containing protein	-
FOC59_RS04130	899651..900562	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
FOC59_RS04135	900671..901270	+	600	WP_003409989.1	L-lysine exporter	-
FOC59_RS04140	901686..902114	+	429	WP_003409992.1	cellulose-binding protein	-
FOC59_RS04145	902340..902879	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
FOC59_RS04150	903399..903959	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
FOC59_RS04155	903956..904297	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
FOC59_RS04160	904383..904640	+	258	WP_003410006.1	hypothetical protein	-
FOC59_RS04165	904541..904876	-	336	WP_003410009.1	dehydrogenase	-
FOC59_RS04170	904965..905309	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
FOC59_RS04175	905303..905551	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
FOC59_RS04180	905651..907966	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
FOC59_RS04185	907963..908235	-	273	WP_003903706.1	DUF1490 family protein	-
FOC59_RS04190	908288..908644	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T143891 WP_003410001.1 NZ_CP046308:c903959-903399 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T143891 NZ_CP061399:2123813-2123915 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT143891 WP_003410003.1 NZ_CP046308:c904297-903956 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT143891 NZ_CP061399:c2123922-2123777 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4