Detailed information of TA system TA143887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 860844..861547
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FOC59_RS03860
• Protein ID: WP_003409778.1
• Coordinates: 860844..861173 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FOC59_RS03865
• Protein ID: WP_003409780.1
• Coordinates: 861170..861547 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS03840	857227..858297	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FOC59_RS03845	858294..858809	+	516	WP_003409718.1	flavin reductase family protein	-
FOC59_RS03850	858806..859867	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FOC59_RS03855	859864..860634	+	771	WP_003409775.1	SDR family oxidoreductase	-
FOC59_RS03860	860844..861173	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOC59_RS03865	861170..861547	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FOC59_RS03870	861544..862134	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FOC59_RS03875	862189..863553	+	1365	WP_003903691.1	HNH endonuclease	-
FOC59_RS03880	863708..864160	-	453	WP_003899095.1	lipoprotein	-
FOC59_RS03885	864224..864625	+	402	WP_003409869.1	hypothetical protein	-
FOC59_RS03890	864618..864800	-	183	WP_003409870.1	hypothetical protein	-
FOC59_RS03895	864914..865264	-	351	WP_003409871.1	hypothetical protein	-
FOC59_RS03900	865275..866177	-	903	WP_003409874.1	hypothetical protein	-
FOC59_RS03905	866198..866389	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T143887 WP_003409778.1 NZ_CP046308:c861173-860844 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T143887 NZ_CP061399:327144-327512 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT143887 WP_003409780.1 NZ_CP046308:c861547-861170 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT143887 NZ_CP061399:326926-327147 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8