Detailed information of TA system TA143883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 338753..339369
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TJ74
• Locus tag: FOC59_RS01535
• Protein ID: WP_003407593.1
• Coordinates: 339052..339369 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TJ73
• Locus tag: FOC59_RS01530
• Protein ID: WP_003900349.1
• Coordinates: 338753..339055 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS01520	334639..336486	+	1848	WP_003407585.1	methylmalonyl-CoA mutase small subunit	-
FOC59_RS01525	336487..338739	+	2253	WP_003407587.1	methylmalonyl-CoA mutase	-
FOC59_RS01530	338753..339055	+	303	WP_003900349.1	type II toxin-antitoxin system antitoxin MazE4	Antitoxin
FOC59_RS01535	339052..339369	+	318	WP_003407593.1	type II toxin-antitoxin system toxin endoribonuclease MazF4	Toxin
FOC59_RS01540	339366..340370	+	1005	WP_003407596.1	methylmalonyl Co-A mutase-associated GTPase MeaB	-
FOC59_RS01545	340423..341712	+	1290	WP_003407599.1	serine hydrolase	-
FOC59_RS01550	341785..342558	-	774	WP_003912632.1	class I SAM-dependent methyltransferase	-
FOC59_RS01555	342616..342828	-	213	WP_003898900.1	dodecin family protein	-
FOC59_RS20855	342838..343062	-	225	WP_160522526.1	NUDIX domain-containing protein	-
FOC59_RS01565	343332..344360	+	1029	WP_003903478.1	glycosyltransferase family 2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11377.97 Da
• Isoelectric Point: 7.9950

>T143883 WP_003407593.1 NZ_CP046308:339052-339369 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Nucleotide

• Length: 318 bp

>T143883 NZ_CP061396:c4973870-4973586 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11173.42 Da
• Isoelectric Point: 9.5564

>AT143883 WP_003900349.1 NZ_CP046308:338753-339055 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Nucleotide

• Length: 303 bp

>AT143883 NZ_CP061396:c4974102-4973860 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 5XE3
• PDB: 5XE2

Antitoxin

• AlphaFold DB: G0TJ73