Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2831517..2832014 Replicon chromosome
Accession NZ_CP046286
Organism Shigella sonnei strain FDAARGOS_715

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FOC23_RS15235 Protein ID WP_001173343.1
Coordinates 2831841..2832014 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FOC23_RS15230 Protein ID WP_000398619.1
Coordinates 2831517..2831813 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC23_RS15210 (2828248) 2828248..2828967 - 720 WP_000611285.1 two-component system response regulator DcuR -
FOC23_RS15215 (2828964) 2828964..2830595 - 1632 WP_001216495.1 sensor histidine kinase -
FOC23_RS15220 (2830776) 2830776..2831006 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
FOC23_RS15225 (2831018) 2831018..2831290 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
FOC23_RS15230 (2831517) 2831517..2831813 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FOC23_RS15235 (2831841) 2831841..2832014 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FOC23_RS15240 (2832133) 2832133..2833650 - 1518 WP_001295086.1 lysine--tRNA ligase -
FOC23_RS15245 (2833887) 2833887..2835344 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
FOC23_RS15250 (2835403) 2835403..2835660 - 258 Protein_2764 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T143820 WP_001173343.1 NZ_CP046286:2831841-2832014 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T143820 NZ_CP061376:c143906-143625 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT143820 WP_000398619.1 NZ_CP046286:2831517-2831813 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT143820 NZ_CP061376:c144130-143903 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References