Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 32093..32628 Replicon plasmid unnamed1
Accession NZ_CP046285
Organism Shigella sonnei strain FDAARGOS_715

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag FOC23_RS00185 Protein ID WP_000222766.1
Coordinates 32341..32628 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0I0JNN8
Locus tag FOC23_RS00180 Protein ID WP_001132903.1
Coordinates 32093..32344 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC23_RS00140 (FOC23_00140) 27359..27820 + 462 WP_001233865.1 thermonuclease family protein -
FOC23_RS00145 (FOC23_00145) 27866..28075 + 210 WP_001299729.1 hemolysin expression modulator Hha -
FOC23_RS00150 (FOC23_00150) 28114..28359 + 246 Protein_27 hypothetical protein -
FOC23_RS00160 (FOC23_00160) 29128..29481 + 354 Protein_29 DUF2726 domain-containing protein -
FOC23_RS00165 (FOC23_00165) 29721..29981 + 261 WP_000083819.1 replication regulatory protein RepA -
FOC23_RS00170 (FOC23_00170) 30205..30279 + 75 WP_001365705.1 RepA leader peptide Tap -
FOC23_RS00175 (FOC23_00175) 30272..31129 + 858 WP_000130974.1 incFII family plasmid replication initiator RepA -
FOC23_RS25560 31829..32020 + 192 WP_000829591.1 hypothetical protein -
FOC23_RS00180 (FOC23_00180) 32093..32344 + 252 WP_001132903.1 plasmid stabilization protein Antitoxin
FOC23_RS00185 (FOC23_00185) 32341..32628 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOC23_RS00190 (FOC23_00190) 32861..34060 - 1200 WP_000947184.1 IS91 family transposase -
FOC23_RS00195 (FOC23_00195) 34070..34258 - 189 WP_000957853.1 hypothetical protein -
FOC23_RS00200 (FOC23_00200) 34380..34538 + 159 Protein_38 transposase -
FOC23_RS00205 (FOC23_00205) 34677..34832 + 156 Protein_39 IS256 family transposase -
FOC23_RS00210 (FOC23_00210) 34862..36589 - 1728 WP_000597732.1 T3SS effector E3 ubiquitin-protein ligase IpaH1.4 -
FOC23_RS00215 (FOC23_00215) 36980..37199 - 220 Protein_41 hypothetical protein -
FOC23_RS00220 (FOC23_00220) 37356..37453 - 98 Protein_42 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH1.4 / tviC / nleE / icsP/sopA / ospB / ospC3 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..213386 213386
- inside IScluster/Tn - ipaH1.4 19847..40862 21015


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T143814 WP_000222766.1 NZ_CP046285:32341-32628 [Shigella sonnei]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T143814 NZ_CP061375:339347-339598 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9225.44 Da        Isoelectric Point: 4.0927

>AT143814 WP_001132903.1 NZ_CP046285:32093-32344 [Shigella sonnei]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT143814 NZ_CP061375:339042-339320 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0I0JNN8

References