Detailed information of TA system TA143809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 74753..75550
• Replicon: plasmid pE70-A
• Accession: NZ_CP046272
• Organism: Enterobacter hormaechei strain E70

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GMA21_RS22265
• Protein ID: WP_172754447.1
• Coordinates: 74753..75256 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: GMA21_RS22270
• Protein ID: WP_016582626.1
• Coordinates: 75281..75550 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMA21_RS22245	72423..73211	+	789	WP_040110304.1	receptor-recognizing protein	-
GMA21_RS22250	73286..73609	+	324	WP_000064173.1	hypothetical protein	-
GMA21_RS22255	73623..74315	+	693	WP_006812500.1	membrane protein	-
GMA21_RS23390	74317..74568	+	252	WP_000147960.1	hypothetical protein	-
GMA21_RS23395	74519..74725	+	207	WP_080399007.1	hypothetical protein	-
GMA21_RS22265	74753..75256	-	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
GMA21_RS22270	75281..75550	-	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
GMA21_RS22275	75967..76632	+	666	WP_023315962.1	AAA family ATPase	-
GMA21_RS22280	76638..76991	+	354	WP_160956455.1	hypothetical protein	-
GMA21_RS22285	77033..77830	-	798	WP_077990606.1	DUF2971 domain-containing protein	-
GMA21_RS22290	78094..78819	+	726	WP_058652463.1	hypothetical protein	-
GMA21_RS22295	78880..80220	+	1341	WP_006812582.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114701	114701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T143809 WP_172754447.1 NZ_CP046272:c75256-74753 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T143809 NZ_CP061369:c3353004-3352825 [Komagataeibacter rhaeticus]
ATGAAGAGTGCCGATCTGATCCGGGAGCTGGAAAAAGCAGGCTGGAAGCTGGATCGCGTGCGCGGTTCACACAACGTCTT
CAAACATCCGTCGCGACCAGGGATCGTGGTCGTCCCTCATCCCAAAAAGGATCTGGGCACCGGTCTGGTTGCCGCAATCC
GCAAACAGGCGGGTCTCTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT143809 WP_016582626.1 NZ_CP046272:c75550-75281 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT143809 NZ_CP061369:c3352814-3352398 [Komagataeibacter rhaeticus]
ATGCGCTATCCGGTTGTTATCGAACGGGGCAGCGAGGCAACGGCATTTGGTGTTGTCTTTCCTGACCTTCCCGGCTGCTT
TTCGGCAGGCGACACGCTCGATGAAGCCATTTCCAATGCCGAAGAGGCCGCTGCGGCATGGATCGATGCCACGCTGGATG
CCGGAGAGGCTGTGCCTTCTCCCAGCACGCTCGACGCCATCCAGAACAACCCGGATTATGAGGGCTGGACAGTGGGCTTT
GTCACCATTGATCCGGCCGTCCTCGATGACCGGGTGGAACGGGCGAACATCTCCCTTCCCCGGCGCGTGCTGGAGCGGTT
GGATGCCCTTGCCAGATCATCCCATGAGAGCCGTTCCGGCATGATCGCGGCCATGACCCTGCAGGCACGGCTTAAGCCGG
TGGTCAGAAGCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3